Mouse anti-Rat, Human CD105 Monoclonal Antibody | anti-CD105 antibody

Anti-Hu CD105 Alexa Fluor488

• Gene Names: ENG; END; ORW; HHT1; ORW1; CD105
• Reactivity: Rat, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD105; Monoclonal Antibody; Anti-Hu CD105 Alexa Fluor488; Endoglin; END; ENG; HHT1; ORW1; endoglin; anti-CD105 antibody

• Host: Mouse
• Reactivity: Rat, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: MEM-226
• Specificity: The antibody MEM-226 reacts with an extracellular epitope of CD105 (Endoglin), a 90kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD105 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor488
• Immunogen: Recombinant Vaccinia virus containing the human CD105 cDNA.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of HUVEC (human umbilical vein endothelial cells) and HEK-293 cells with anti-CD105 (MEM-226) Alexa Fluor 488.)

Related Product Information for anti-CD105 antibody

CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

References

*Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116(11 Suppl):I64-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/17846327,*Asumda FZ, Chase PB: Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol. 2011 Oct 12;12:44., URL: https://www.ncbi.nlm.nih.gov/pubmed/21992089
*Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12947156ordin,*Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/12746487ordin,*Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/15274293ordin,*Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional Application (FUNC)tional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16158917ordin,*Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16733295ordin

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1
• NCBI GenBank Nucleotide #: NM_000118.2
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 70,578 Da
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; ORW; HHT1; ORW1; CD105
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN