Rabbit ARF6 Polyclonal Antibody | anti-ARF6 antibody

ARF6 Polyclonal Antibody

Cat. Num. AAA3005687

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARF6; Polyclonal Antibody; ARF6 Polyclonal Antibody; ADP ribosylation factor 6; ADP ribosylation factor protein 6; ADP-ribosylation factor 6; anti-ARF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARF6 polyclonal antibody detects endogenous levels of ARF6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 175

• Applicable Applications for anti-ARF6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 114-159 of Human ARF6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARF6 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat spleen tissue lysate)

Related Product Information for anti-ARF6 antibody

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4502211
• NCBI GeneID: 382
• NCBI Accession #: NP_001654.1
• NCBI GenBank Nucleotide #: NP_001654.1
• UniProt Accession #: P62330
• Molecular Weight: ~ 20, 28kDa
• NCBI Official Full Name: ADP-ribosylation factor 6
• NCBI Official Synonym Full Names: ADP ribosylation factor 6
• NCBI Official Symbol: ARF6
• NCBI Protein Information: ADP-ribosylation factor 6
• UniProt Protein Name: ADP-ribosylation factor 6
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF6
• UniProt Entry Name: ARF6_HUMAN

NCBI Description

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: Golgi apparatus; ruffle; filopodium membrane; focal adhesion; membrane; endocytic vesicle; recycling endosome membrane; early endosome; plasma membrane; cell cortex; midbody; endosome; cleavage furrow

Molecular Function: GTPase activity; protein binding; GTP binding; thioesterase binding

Biological Process: myeloid cell apoptosis; metabolic process; regulation of filopodium formation; liver development; cell cycle; vesicle-mediated transport; regulation of Rac protein signal transduction; protein transport; positive regulation of actin filament polymerization; cell division; small GTPase mediated signal transduction; ruffle organization and biogenesis; negative regulation of receptor-mediated endocytosis; cortical actin cytoskeleton organization and biogenesis; cell adhesion; cell motility