ARF6 Antibody | anti-ARF6 antibody

ARF6 Antibody

Cat. Num. AAA8516087

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: ARF6; ARF6 Antibody; DKFZp564M0264; anti-ARF6 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: ARF6 Antibody detects endogenous levels of total ARF6
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 175

• Applicable Applications for anti-ARF6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ARF6
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of A431 cell and 293T cell lysate using ART6 antibody.)

Related Product Information for anti-ARF6 antibody

ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily (1). Arf proteins are grouped into three distinct classes based on amino acid sequence and structural similarity, with Arf6 as the single class III protein to date. Arf6 is localized mainly to the plasma membrane and endosomes (1, 2). This small GTPase interacts with PIP5K, PLD and Rac1, proteins important in lipid metabolism and actin regulation. Arf6 function depends upon its cycling between GDP-and GTP-bound states, which is regulated by associated GAP and GEF factors (3, 4). Plasma membrane-associated Arf6 appears to play several functions during the many steps of membrane trafficking, including regulating membrane receptor internalization in both clathrin-dependent and independent pathways, endosomal recycling, and proximal actin reorganization and remodeling (5, 6).

NCBI and Uniprot Product Information

• NCBI GI #: 4502211
• NCBI GeneID: 382
• NCBI Accession #: NP_001654.1
• NCBI GenBank Nucleotide #: NM_001663.3
• UniProt Accession #: P62330; P26438; Q6FGZ2
• Molecular Weight: 20kDa
• NCBI Official Full Name: ADP-ribosylation factor 6
• NCBI Official Synonym Full Names: ADP ribosylation factor 6
• NCBI Official Symbol: ARF6
• NCBI Protein Information: ADP-ribosylation factor 6
• UniProt Protein Name: ADP-ribosylation factor 6
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF6

NCBI Description

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis (). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (). May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 ().