Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ARD1A Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ARD1A Polyclonal Antibody | anti-ARD1A antibody
ARD1A Antibody
Predicted: Pig, Bovine, Horse, Sheep, Rabbit
Predicted: Pig, Bovine, Horse, Sheep, Rabbit
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ARD1A Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ARD1A Antibody. The lane on the left was treated with blocking peptide.)
Post Translational Modifications: Cleaved by caspases during apoptosis. Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation. Autoacetylated at Lys-136 which stimulates its catalytic activity.
Subunit Structure: Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA10 and NAA15 or NAA16 (PubMed:20154145). Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, an down-regulates it when induced by hypoxia (PubMed:12464182). Interacts with NAA50 and with the ribosome (PubMed:16507339). Binds to MYLK (PubMed:19826488). Associates with HYPK when in complex with NAA15 (PubMed:15496142). Interacts with NAA15 (PubMed:15496142). Interacts with NAA16 (PubMed:19480662). Interacts (via its C-terminal domain) with TSC2, leading to its acetylation (PubMed:20145209). Interacts with NAA16 (PubMed:19480662). Interacts with IKBKB (PubMed:19716809). Interacts with HSPA1A and HSPA1B leading to its acetylation (PubMed:27708256).
Similarity: Belongs to the acetyltransferase family. ARD1 subfamily.
NCBI and Uniprot Product Information
Predicted: 27 kDa
NCBI Description
N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily.
Protein type: Amino Acid Metabolism - tyrosine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; EC 2.3.1.88; Lipid Metabolism - glycerophospholipid; Acetyltransferase
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: membrane; cytoplasm; nucleolus; intracellular; nucleus
Molecular Function: peptide alpha-N-acetyltransferase activity; N-acetyltransferase activity; protein binding; acetyltransferase activity; ribosome binding
Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; internal protein amino acid acetylation; N-terminal peptidyl-serine acetylation; DNA packaging
Disease: Ogden Syndrome; Microphthalmia, Syndromic 1
Research Articles on ARD1A
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Product Notes
The ARD1A naa10 (Catalog #AAA9611579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARD1A Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's ARD1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ARD1A naa10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARD1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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