Rabbit anti-Human NAA10 Polyclonal Antibody | anti-NAA10 antibody

NAA10 Antibody (Center)

Cat. Num. AAA9216881

• Gene Names: NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: NAA10; Polyclonal Antibody; NAA10 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); N-alpha-acetyltransferase 10; 2.3.1.-; 2.3.1.88; N-terminal acetyltransferase complex ARD1 subunit homolog A; NatA catalytic subunit Naa10; ARD1; ARD1A; TE2; anti-NAA10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Sequence Length: 220

• Applicable Applications for anti-NAA10 antibody: Western Blot (WB)
• Application Notes: WB~~1:2000
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NAA10 Antibody (Center) at 1:2000 dilutionLane 1: SK-BR-3 whole cell lysateLane 2: Hela whole cell lysateLane 3: HCT116 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NAA10 antibody

Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development.

Product Categories/Family for anti-NAA10 antibody

Crown Antibodies; Metabolism; Signal Transduction

References

Tribioli C.,et al.Hum. Mol. Genet. 3:1061-1068(1994). Arnesen T.,et al.Biochem. J. 386:433-443(2005). Ross M.T.,et al.Nature 434:325-337(2005). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Jeong J.-W.,et al.Cell 111:709-720(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 371121601
• NCBI GeneID: 8260
• NCBI Accession #: NP_001243048.1
• NCBI GenBank Nucleotide #: NM_001256119.1
• UniProt Accession #: P41227; A6NM98
• Molecular Weight: 24,784 Da
• NCBI Official Full Name: N-alpha-acetyltransferase 10 isoform 2
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 10, NatA catalytic subunit
• NCBI Official Symbol: NAA10
• NCBI Official Synonym Symbols: TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
• NCBI Protein Information: N-alpha-acetyltransferase 10
• UniProt Protein Name: N-alpha-acetyltransferase 10
• Protein Family: N-alpha-acetyltransferase
• UniProt Gene Name: NAA10
• UniProt Synonym Gene Names: ARD1; ARD1A; TE2
• UniProt Entry Name: NAA10_HUMAN

NCBI Description

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily.

Protein type: Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - tyrosine; EC 2.3.1.88; Amino Acid Metabolism - phenylalanine; Acetyltransferase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; intracellular; membrane; nucleolus; nucleus

Molecular Function: acetyltransferase activity; N-acetyltransferase activity; peptide alpha-N-acetyltransferase activity; protein binding; ribosome binding

Biological Process: DNA packaging; internal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; N-terminal peptidyl-serine acetylation; N-terminal protein amino acid acetylation

Disease: Microphthalmia, Syndromic 1; Ogden Syndrome