Western Blot (WB)
(Anti- 12 Lipoxygenase antibody, MBS177437, Western blottingAll lanes: Anti ANOX12 (MBS177437) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 75KDObserved bind size: 75KD )
Rabbit Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase) Polyclonal Antibody | anti-ALOX12 antibody
Anti-12 Lipoxygenase antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Western Blot (WB)
(Anti- 12 Lipoxygenase antibody, MBS177437, Western blottingAll lanes: Anti ANOX12 (MBS177437) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 75KDObserved bind size: 75KD )
Western Blot (WB)
(Anti- 12 Lipoxygenase antibody, MBS177437, Western blottingAll lanes: Anti ANOX12 (MBS177437) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 75KDObserved bind size: 75KD )
Background: ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.
NCBI and Uniprot Product Information
Uniprot Description
ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.
Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.31; Oxidoreductase; Apoptosis
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: membrane; cytoplasm; cytosol; sarcolemma
Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; protein binding; lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; positive regulation of caspase activity; linoleic acid metabolic process; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; lipoxygenase pathway; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; hepoxilin metabolic process; superoxide release; aging; positive regulation of cell migration; negative regulation of apoptosis
Research Articles on ALOX12
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Product Notes
The ALOX12 alox12 (Catalog #AAA177437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-12 Lipoxygenase antibody reacts with Human. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human. Researchers should empirically determine the suitability of the ALOX12 alox12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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