Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit | ALOX12 elisa kit

Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit

Cat. Num. AAA2000243

• Gene Names: ALOX12; LOG12; 12-LOX; 12S-LOX
• Reactivity: Human
• Synonyms: Arachidonate-12-Lipoxygenase (ALOX12); Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit; LOX12; 12-LOX; ALOX-12; 12-LO; 12LPG; LOG12; Lipoxygenase 12; 12S-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12; ALOX12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12).; No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed.
• Sequence Length: 663

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ALOX12 elisa kit

Enzyme & Kinase; Metabolic pathway; Neuroscience; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 154426292
• NCBI GeneID: 239
• NCBI Accession #: NP_000688.2
• NCBI GenBank Nucleotide #: NM_000697.2
• UniProt Accession #: P18054; O95569; Q6ISF8; Q9UQM4
• Molecular Weight: 75,694 Da
• NCBI Official Full Name: arachidonate 12-lipoxygenase, 12S-type
• NCBI Official Synonym Full Names: arachidonate 12-lipoxygenase
• NCBI Official Symbol: ALOX12
• NCBI Official Synonym Symbols: LOG12; 12-LOX; 12S-LOX
• NCBI Protein Information: arachidonate 12-lipoxygenase, 12S-type
• UniProt Protein Name: Arachidonate 12-lipoxygenase, 12S-type
• Protein Family: Arachidonate 12-lipoxygenase
• UniProt Gene Name: ALOX12
• UniProt Synonym Gene Names: 12LO; LOG12; 12S-LOX; 12S-lipoxygenase
• UniProt Entry Name: LOX12_HUMAN

Uniprot Description

ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; Apoptosis; EC 1.13.11.31

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; sarcolemma

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; lipoxygenase activity; iron ion binding

Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; positive regulation of caspase activity; linoleic acid metabolic process; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; lipoxygenase pathway; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; hepoxilin metabolic process; superoxide release; negative regulation of apoptosis; positive regulation of cell migration; aging