Immunohistochemistry (IHC)
(Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Rabbit anti-Human ALOX12 / 12 Lipoxygenase Polyclonal Antibody | anti-ALOX12 antibody
Anti-ALOX12 / 12 Lipoxygenase Antibody (aa618-650) IHC-plus
IF (1:10 - 1:50)
IHC-P (1:50)
WB (1:1000)
For long term storage store at -20 degree C.
Immunohistochemistry (IHC)
(Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Immunohistochemistry (IHC)
(Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Immunofluorescence (IF)
(Immunofluorescence of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Immunofluorescence (IF)
(Immunofluorescence of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Western Blot (WB)
(Western blot of ALOX12 Antibody in K562 cell line lysates (35 ug/lane). ALOX12 (arrow) was detected using the purified antibody.)
Western Blot (WB)
(Western blot of ALOX12 Antibody in K562 cell line lysates (35 ug/lane). ALOX12 (arrow) was detected using the purified antibody.)
Flow Cytometry (FC/FACS)
(ALOX12 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ALOX12 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.
Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.13.11.31; Apoptosis
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: cytoplasm; cytosol; membrane; sarcolemma
Molecular Function: arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; lipoxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding
Biological Process: aging; arachidonic acid metabolic process; cell motility; fatty acid oxidation; hepoxilin biosynthetic process; hepoxilin metabolic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; positive regulation of smooth muscle cell proliferation; positive regulation of vasodilation; superoxide release
Research Articles on ALOX12
Similar Products
Product Notes
The ALOX12 alox12 (Catalog #AAA2400174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALOX12 / 12 Lipoxygenase Antibody (aa618-650) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX12 / 12 Lipoxygenase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:10 - 1:50) IF (1:10 - 1:50) IHC-P (1:50) WB (1:1000). Researchers should empirically determine the suitability of the ALOX12 alox12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX12 / 12 Lipoxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
