Chicken anti-Rat Aquaporin 8 Polyclonal Antibody | anti-AQP8 antibody

Aquaporin 8

Cat. Num. AAA620888

• Gene Names: AQP8; AQP-8
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Aquaporin 8; Polyclonal Antibody; Anti -Aquaporin 8; anti-AQP8 antibody

• Host: Chicken
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: The 16aa peptide was found unique to AQP8 without significant homology to any other AQPs. It has 93% homology with the mouse AQP8. AQP7 from other species are yet to be cloned.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS before the addition of glycerol to 40%.

• Applicable Applications for anti-AQP8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500-1:2500; ELISA: 1:5000-25,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: A 16aa synthetic peptide within the C-terminal cytoplasmic domain of rat AQP8.
• Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-AQP8 antibody

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. A new water channel, AQP8, has been identified in rat pancreas and testis by homology cloning (1). AQP8 (263 aa; 28kD) is also found in liver, colon and salivary glands. AQP8 is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport.

Product Categories/Family for anti-AQP8 antibody

Antibodies; Abs to Aquaporin

NCBI and Uniprot Product Information

• NCBI GI #: 6606076
• NCBI GeneID: 343
• UniProt Accession #: O94778; Q8IUU3; Q9UIA4; Q53GF6
• Molecular Weight: 27,381 Da
• NCBI Official Full Name: aquaporin 8
• NCBI Official Synonym Full Names: aquaporin 8
• NCBI Official Symbol: AQP8
• NCBI Official Synonym Symbols: AQP-8
• NCBI Protein Information: aquaporin-8; OTTHUMP00000122504
• UniProt Protein Name: Aquaporin-8
• Protein Family: Aquaporin
• UniProt Gene Name: AQP8
• UniProt Entry Name: AQP8_HUMAN

NCBI Description

Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq]

Uniprot Description

AQP8: Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Channel, misc.; Transporter, aquaporin family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical part of cell; plasma membrane

Molecular Function: water channel activity; glycerol channel activity

Biological Process: glycerol transport; response to reactive oxygen species; cellular water homeostasis; transport; canalicular bile acid transport; water transport; transmembrane transport