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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Apolipoprotein O Polyclonal Antibody | anti-APOO antibody

Polyclonal Antibody to Apolipoprotein O (APOO)

Gene Names
APOO; MIC26; Mic23; My025; FAM121B
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Apolipoprotein O; Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein O (APOO); anti-APOO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against APOO. It has beenselected for its ability to recognize APOO in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-APOO antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
APOO (Lys26~Lys198)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant APOO (Lys26~Lys198) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072700
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,199 Da
NCBI Official Full Name
MICOS complex subunit MIC26
NCBI Official Synonym Full Names
apolipoprotein O
NCBI Official Symbol
APOO
NCBI Official Synonym Symbols
MIC26; Mic23; My025; FAM121B
NCBI Protein Information
MICOS complex subunit MIC26
UniProt Protein Name
MICOS complex subunit MIC26
Protein Family
UniProt Gene Name
APOO

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

APOO: Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation. Belongs to the apolipoprotein O family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: cytosol; endoplasmic reticulum membrane; extracellular region; extracellular space; Golgi membrane; integral to mitochondrial inner membrane; mitochondrion

Molecular Function: protein binding

Biological Process: cristae formation; lipid transport

Research Articles on APOO

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Product Notes

The APOO apoo (Catalog #AAA2026905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apolipoprotein O (APOO) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein O can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the APOO apoo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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