anti-Human, Mouse ASK1 Antibody | anti-ASK1 antibody

ASK1 (Ab-966) Antibody

Cat. Num. AAA856880

• Gene Names: MAP3K5; ASK1; MEKK5; MAPKKK5
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ASK1; ASK1 (Ab-966) Antibody; anti-ASK1 antibody

• Reactivity: Human, Mouse
• Specificity: ASK1 (Ab-966) antibody detects endogenous levels of total ASK1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1374

• Applicable Applications for anti-ASK1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:100
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 (Ab-966) antibody.)

Product Categories/Family for anti-ASK1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044. Fujii K, et al. (2004) Oncogene. 23(29):5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.

NCBI and Uniprot Product Information

• NCBI GI #: 5174547
• NCBI GeneID: 4217
• NCBI Accession #: NP_005914.1
• NCBI GenBank Nucleotide #: NM_005923.3
• UniProt Accession #: Q99683; Q5THN3; Q99461; A6NIA0; B4DGB2
• Molecular Weight: 69,839 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: MAP3K5
• NCBI Official Synonym Symbols: ASK1; MEKK5; MAPKKK5
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: DASH complex
• UniProt Gene Name: MAP3K5
• UniProt Synonym Gene Names: ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5

NCBI Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).