Rabbit anti-Rat Apolipoprotein B (APOB) Polyclonal Antibody | anti-APOB antibody

Polyclonal Antibody to Apolipoprotein B (APOB)

Cat. Num. AAA2004451

• Gene Names: Apob; Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Apolipoprotein B (APOB); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein B (APOB); anti-APOB antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with a His-tag and its sequence is listed below.; MGHHHHHHSGS-TNKFLK GSHDSTISLT KKNMEASVKT TANLHAPIFT MNFKQELNGN TKSKPTVSSS IELNYDFNSS KLHSAAKGGV DHKFSLESLT SYLSIESFTK GNIKGSFLSQ EYSGSVANEA NVYLNSKGTR SSVRLQGASN FAGIWNFEVG ENFAGEATLR RIYGTWEHNM INHLQVFSYF DTKGKQTCRA
• Sequence Length: 1670

• Applicable Applications for anti-APOB antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant APOB (Thr3365~Ala3550) expressed in E.coli.
• Cross Reactivity: Human, Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2061922
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat APOB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 161783809
• NCBI GeneID: 54225
• NCBI Accession #: NP_062160.2
• NCBI GenBank Nucleotide #: NM_019287.2
• UniProt Accession #: Q7TMA5
• Molecular Weight: 536,024 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q14

Cellular Component: cell soma; chylomicron; cytoplasm; cytosol; endoplasmic reticulum; extracellular space; intracellular membrane-bound organelle; vesicle membrane

Molecular Function: cholesterol transporter activity; heparin binding; lipid binding; lipid transporter activity; low-density lipoprotein receptor binding; phospholipid binding; protein binding

Biological Process: artery morphogenesis; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fertilization; in utero embryonic development; lipid catabolic process; lipid metabolic process; lipid transport; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; post-embryonic development; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to estradiol; response to lipopolysaccharide; response to organic substance; response to selenium ion; response to virus; sperm motility; spermatogenesis; triacylglycerol mobilization; triglyceride catabolic process