Mouse anti-Human CIB1 Monoclonal Antibody | anti-CIB1 antibody

Anti-CIB1 Antibody

Cat. Num. AAA418379

• Gene Names: CIB1; CIB; CIBP; KIP1; PRKDCIP; SIP2-28
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: CIB1; Monoclonal Antibody; Anti-CIB1 Antibody; anti-CIB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 231

• Applicable Applications for anti-CIB1 antibody: Western Blot (WB)
• Immunogen: Ni-NTA purified truncated recombinant CIB1 expressed in E Coli strain BL21 (DE3)
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-CIB1 antibody

CIB1 (also designated calcium and integrin binding 1 or calmyrin), with 191-amino acid protein (about 21kDa), belongs to the calcium-binding protein family. CIB1 is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.CIB1 is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alpha (IIb)beta (3) integrin and several serine/threonine kinases and potentially modulates their function.CIB1 regulates platelet aggregation in hemostasis through a specific interaction with the alpha (IIb) cytoplasmic domain of platelet integrin alpha (IIb)beta (3). CIB1 is also ubiquitously expressed activating and inhibiting protein ligand of the InsP3R.

References

1. Holly R. Gentry, Alex U. Singer, Laurie Betts J. Biol. Chem., Mar 2005; 280: 8407 - 8415. 2. Carl White, Jun Yang, Mervyn J. Monteiro J. Biol. Chem., Jul 2006; 281: 20825 - 20833 3. Aaron P. Yamniuk, Hiroaki Ishida, Hans J. Vogel J. Biol. Chem., Jul 2006; 10.1074/jbc.M603963200.

NCBI and Uniprot Product Information

• NCBI GI #: 480540325
• NCBI GeneID: 10519
• NCBI Accession #: NP_001264693.1
• NCBI GenBank Nucleotide #: NM_001277764.1
• UniProt Accession #: Q99828; O00693; O00735; Q6IB49; Q96J54; Q99971; B5BU40; H6WJF3
• Molecular Weight: 26,032 Da
• NCBI Official Full Name: calcium and integrin-binding protein 1 isoform a
• NCBI Official Synonym Full Names: calcium and integrin binding 1
• NCBI Official Symbol: CIB1
• NCBI Official Synonym Symbols: CIB; CIBP; KIP1; PRKDCIP; SIP2-28
• NCBI Protein Information: calcium and integrin-binding protein 1
• UniProt Protein Name: Calcium and integrin-binding protein 1
• Protein Family: Calcium and integrin-binding protein
• UniProt Gene Name: CIB1
• UniProt Synonym Gene Names: CIB; KIP; PRKDCIP; CIB; CIBP; KIP; SIP2-28

NCBI Description

This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. The encoded protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. The encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CIB1: May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cell soma; centrosome; cytoplasm; endoplasmic reticulum; filopodium tip; growth cone; lamellipodium; membrane; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; sarcolemma; vesicle

Molecular Function: calcium ion binding; calcium-dependent protein kinase inhibitor activity; protein anchor; protein binding; protein serine/threonine kinase inhibitor activity; Ras GTPase binding

Biological Process: activation of NF-kappaB transcription factor; apoptosis; cytoplasmic microtubule organization and biogenesis; double-strand break repair; endomitotic cell cycle; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of megakaryocyte differentiation; negative regulation of microtubule depolymerization; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; platelet formation; positive regulation of catalytic activity; positive regulation of cell adhesion mediated by integrin; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of protein amino acid phosphorylation; regulation of cell division; regulation of cell proliferation; response to DNA damage stimulus; spermatid development