Apolipoprotein B (APOB) Recombinant Protein | APOB recombinant protein

Recombinant Apolipoprotein B (APOB)

Cat. Num. AAA2009239

• Gene Names: Apob; Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apolipoprotein B (APOB); Recombinant Apolipoprotein B (APOB); APOB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-TNKFLK GSHDSTISLT KKNMEASVKT TANLHAPIFT MNFKQELNGN TKSKPTVSSS IELNYDFNSS KLHSAAKGGV DHKFSLESLT SYLSIESFTK GNIKGSFLSQ EYSGSVANEA NVYLNSKGTR SSVRLQGASN FAGIWNFEVG ENFAGEATLR RIYGTWEHNM INHLQVFSYF DTKGKQTCRA
• Sequence Length: 4506

• Applicable Applications for APOB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr3365~Ala3550 (Accession # Q7TMA5) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 161783809
• NCBI GeneID: 54225
• NCBI Accession #: NP_062160.2
• NCBI GenBank Nucleotide #: NM_019287.2
• UniProt Accession #: Q7TMA5
• Molecular Weight: 21.8kDa
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100; apolipoprotein B PI; apolipoprotein B-48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_RAT

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted; Secreted, signal peptide; Carrier

Cellular Component: Golgi apparatus; extracellular space; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; vesicle membrane; actin cytoskeleton

Molecular Function: heparin binding; lipid transporter activity; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding; lipid binding

Biological Process: lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to organic substance; response to selenium ion; triacylglycerol catabolic process; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol homeostasis; sperm motility; fertilization; lipoprotein biosynthetic process; lipoprotein transport; artery morphogenesis; spermatogenesis; lipid metabolic process; triacylglycerol mobilization; lipid catabolic process