Rabbit anti-Human, Mouse APC6 / CDC16 Polyclonal Antibody | anti-APC6 antibody

Rabbit Polyclonal to Human APC6 / CDC16

Cat. Num. AAA243104

• Gene Names: CDC16; APC6; CUT9; ANAPC6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: APC6 / CDC16; Polyclonal Antibody; Rabbit Polyclonal to Human APC6 / CDC16; Anti-APC6 / CDC16 Antibody (N-Terminus) IHC-plus; CDC16; ANAPC6; APC6; CDC16 homolog; Cyclosome subunit 6; CDC16Hs; CUT9; Human APC6; anti-APC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Human CDC16
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 620

• Applicable Applications for anti-APC6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: APC6 antibody can be used for detection of APC6 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: An 18 amino acid peptide near the amino terminus of human APC6.
• Immunogen Type: Synthetic peptide
• Immunogen: APC6 / CDC16 antibody was raised against an 18 amino acid peptide near the amino terminus of human APC6.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CDC16 / Apc6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CDC16 / Apc6 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 118402580
• NCBI GeneID: 8881
• NCBI Accession #: NP_003894.3
• NCBI GenBank Nucleotide #: NM_003903.3
• UniProt Accession #: Q13042; Q5T8C8; Q7Z651; Q96AE6; Q9Y564; A2A365
• Molecular Weight: 60,371 Da
• NCBI Official Full Name: cell division cycle protein 16 homolog
• NCBI Official Synonym Full Names: cell division cycle 16
• NCBI Official Symbol: CDC16
• NCBI Official Synonym Symbols: APC6; CUT9; ANAPC6
• NCBI Protein Information: cell division cycle protein 16 homolog; CDC16Hs; cyclosome subunit 6; cell division cycle 16 homolog; anaphase-promoting complex subunit 6; anaphase-promoting complex, subunit 6
• UniProt Protein Name: Cell division cycle protein 16 homolog
• UniProt Gene Name: CDC16
• UniProt Synonym Gene Names: ANAPC6; APC6; CDC16Hs
• UniProt Entry Name: CDC16_HUMAN

NCBI Description

This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC16: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with PPP5C and CDC20. Belongs to the APC6/CDC16 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; regulation of mitosis; mitotic cell cycle