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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using ANXA11 antibody.)

Rabbit ANXA11 Polyclonal Antibody | anti-ANXA11 antibody

ANXA11 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ANXA11; Polyclonal Antibody; ANXA11 Polyclonal Antibody; Annexin A11; 56 kDa autoantigen; Annexin XI; Annexin-11; Calcyclin-associated annexin 50; CAP-50; ANX11; anti-ANXA11 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ANXA11 polyclonal antibody detects endogenous levels of ANXA11 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
472
Applicable Applications for anti-ANXA11 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IP: 1:50-1:100
Immunogen
Recombinant full length Human ANXA11.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ANXA11 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using ANXA11 antibody.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody.)
Related Product Information for anti-ANXA11 antibody
This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 54kDa
NCBI Official Full Name
annexin A11 isoform 2
UniProt Protein Name
Annexin A11
Protein Family
UniProt Gene Name
ANXA11
UniProt Synonym Gene Names
ANX11; CAP-50
UniProt Entry Name
ANX11_HUMAN

Uniprot Description

ANXA11: a calcium/phospholipid-binding protein found throughout the nucleoplasm at interphase and at the mitotic phase is concentrated at the loop-like structure around the mitotic apparatus. May interact with calcyclin (S100A6) in vivo. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Nuclear envelope; Motility/polarity/chemotaxis; Calcium-binding

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm; specific granule; azurophil granule; membrane; cytoplasm; melanosome; spindle; nuclear envelope; phagocytic vesicle; midbody

Molecular Function: protein binding; phosphatidylethanolamine binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to calcium ion; phagocytosis

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Product Notes

The ANXA11 anxa11 (Catalog #AAA3012091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANXA11 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IF: 1:50-1:200 IP: 1:50-1:100. Researchers should empirically determine the suitability of the ANXA11 anxa11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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