Rabbit ANNEXIN I Polyclonal Antibody | anti-ANXA1 antibody

RABBIT ANTI HUMAN ANNEXIN I

Cat. Num. AAA223575

• Gene Names: ANXA1; ANX1; LPC1
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: ANNEXIN I; Polyclonal Antibody; RABBIT ANTI HUMAN ANNEXIN I; anti-ANXA1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human Annexin I (ANXA1), otherwise known as Lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family, preferentially expressed on the cytosolic face of the plasma membrane, which acts as an inhibitor of phospholipase A2, and may exhibit ; anti-inflammatory activity. ; ; Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive marker.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 346

• Applicable Applications for anti-ANXA1 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 1/3000; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: 6xHis-tagged full-length recombinant human ANXA1 (NP_000691.1).; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human ANXA1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Testing Data #1

(Paraffin embedded human colon carcinoma stained with Rabbit anti human Annexin I (MBS223575))

Related Product Information for anti-ANXA1 antibody

MBS223575 specifically recognises human Annexin I (ANXA1), otherwise known as Lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family, preferentially expressed on the cytosolic face of the plasma membrane, which acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive marker.

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.2
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; annexin I (lipocortin I); annexin-1; calpactin II; calpactin-2; chromobindin-9; phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN

NCBI Description

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Uniprot Description

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: extracellular space; protein complex; cell surface; mast cell granule; focal adhesion; basolateral plasma membrane; extracellular region; cornified envelope; cilium; mitochondrial membrane; cytoplasm; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; helicase activity; calcium-dependent protein binding; single-stranded DNA binding; receptor binding

Biological Process: response to drug; prostate gland development; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; arachidonic acid secretion; neutrophil homeostasis; signal transduction; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; inflammatory response; cell motility; response to X-ray; negative regulation of apoptosis; positive regulation of prostaglandin biosynthetic process