Annexin A1/ANXA1 Protein Recombinant Protein | ANXA1 recombinant protein

Recombinant Human Annexin A1/ANXA1 Protein

Cat. Num. AAA2569269

• Gene Names: ANXA1; ANX1; LPC1
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Annexin A1/ANXA1 Protein; Recombinant Human Annexin A1/ANXA1 Protein; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 Inhibitory Protein; p35; ANXA1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 20mM PB, 150mM NaCl, pH7.2.
• Sequence: Ala2-Asn346

• Species: Human
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Annexin A1 is produced by our E Coli expression system and the target gene encoding Ala2-Asn346 is expressed.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 45 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for ANXA1 recombinant protein

Annexin A1 is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis. Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity. Annexin A1 also has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development. ANXA1 is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumor cells in 97% of samples from patients with hairy cell leukemia.

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.1
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN

NCBI Description

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: extracellular space; protein complex; focal adhesion; cell surface; mast cell granule; basolateral plasma membrane; extracellular region; cornified envelope; cilium; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; protein homodimerization activity; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; calcium ion binding; calcium-dependent protein binding; helicase activity; protein binding; single-stranded RNA binding; double-stranded DNA-dependent ATPase activity; phospholipid binding; structural molecule activity; single-stranded DNA binding; receptor binding

Biological Process: prostate gland development; response to drug; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; positive regulation of vesicle fusion; alpha-beta T cell differentiation; arachidonic acid secretion; neutrophil homeostasis; signal transduction; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; cell motility; inflammatory response; response to X-ray; positive regulation of prostaglandin biosynthetic process; negative regulation of apoptosis