Western Blot (WB)
(ANKRD34B Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ANKRD34B antibody detected the ANKRD34B protein (arrow).)
Rabbit anti-Human ANKRD34B Polyclonal Antibody | anti-Ankrd34b antibody
ANKRD34B, CT (ANKRD34B, Ankyrin repeat domain-containing protein 34B)
Gene Names
Ankrd34b; DP58; 6430502M16Rik
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ANKRD34B; Polyclonal Antibody; CT (ANKRD34B; Ankyrin repeat domain-containing protein 34B); Anti -ANKRD34B; anti-Ankrd34b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Ankrd34b antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Immunogen
ANKRD34B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human ANKRD34B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ANKRD34B Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ANKRD34B antibody detected the ANKRD34B protein (arrow).)
Western Blot (WB)
(ANKRD34B Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ANKRD34B antibody detected the ANKRD34B protein (arrow).)
Immunohistochemistry (IHC)
(ANKRD34B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKRD34B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ANKRD34B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKRD34B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ANKRD34B Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ANKRD34B Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-Ankrd34b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,417 Da
NCBI Official Full Name
Ankrd34b protein
NCBI Official Synonym Full Names
ankyrin repeat domain 34B
NCBI Official Symbol
Ankrd34b
NCBI Official Synonym Symbols
DP58; 6430502M16Rik
NCBI Protein Information
ankyrin repeat domain-containing protein 34B; dendritic protein of 58 kDa; cytosolic phosphoprotein DP58; dendritic phosphoprotein 58 kDa
UniProt Protein Name
Ankyrin repeat domain-containing protein 34B
Protein Family
UniProt Gene Name
Ankrd34b
UniProt Synonym Gene Names
Dp58
UniProt Entry Name
AN34B_MOUSE
Uniprot Description
Subcellular location: Cytoplasm. Nucleus Ref.4.
Tissue specificity: Specifically and constitutively expressed in brain (at protein level). Ref.4
Induction: Up-regulated in bone marrow upon differentiation (at protein level). Ref.3 Ref.4
Post-translational modification: Phosphorylated. Ref.3 Ref.4
Sequence similarities: Belongs to the ANKRD34 family.Contains 4 ANK repeats.
Sequence caution: The sequence AAH96609.1 differs from that shown. Reason: Frameshift at position 199.
Research Articles on Ankrd34b
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Product Notes
The Ankrd34b ankrd34b (Catalog #AAA648452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANKRD34B, CT (ANKRD34B, Ankyrin repeat domain-containing protein 34B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD34B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the Ankrd34b ankrd34b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD34B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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