Rabbit Frizzled-9 Polyclonal Antibody | anti-FZD9 antibody

Frizzled-9 Polyclonal Antibody

Cat. Num. AAA3005859

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Frizzled-9; Polyclonal Antibody; Frizzled-9 Polyclonal Antibody; Frizzled9; Frizzled 9; Frizzled3; Frizzled 3; Frizzled-3; Fz-9; hFz9; FzE6; CD349; FZD9; FZD3; anti-FZD9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Frizzled-9 polyclonal antibody detects endogenous levels of Frizzled-9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 591

• Applicable Applications for anti-FZD9 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 571-620 of Human Frizzled-9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Frizzled-9 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-FZD9 antibody

The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The frizzled-9 gene is located within the Williams Syndrome common deleted region at chromosomal band 7q11.23. Heterozygous deletion of the frizzled-9 gene may contribute to the Williams Syndrome phenotype. In mouse, frizzled-9 overexpression can induce the hyperphosphorylation and relocalization of Dvl-1 from the cytoplasm to the cell membrane and cytosolic beta-catenin accumulation. In rat, frizzled-9 is important in Wnt/beta-catenin signaling in 293T cells. Frizzled-9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NP_003499.1
• UniProt Accession #: O00144
• Molecular Weight: ~ 64kDa
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled class receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9
• UniProt Protein Name: Frizzled-9
• Protein Family: Frizzled
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9
• UniProt Entry Name: FZD9_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: filopodium membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein homodimerization activity; protein heterodimerization activity

Biological Process: nervous system development; G-protein coupled receptor protein signaling pathway; learning and/or memory; B cell differentiation; neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin