Western Blot (WB)
(Western Blot (WB) analysis of Angptl1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat spleen tissue lysate)
Rabbit Angptl1 Polyclonal Antibody | anti-Angptl1 antibody
Angptl1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Angptl1; Polyclonal Antibody; Angptl1 Polyclonal Antibody; Angiopoietin-related protein 1; Angiopoietin-3; ANG-3; Angiopoietin-like protein 1; ANGPTL1; ANG3; ANGPT3; ARP1; PSEC0154; UNQ162/PRO188; anti-Angptl1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Angptl1 polyclonal antibody detects endogenous levels of Angptl1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
491
Applicable Applications for anti-Angptl1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 58-103 of Human Angptl1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Angptl1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat spleen tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Angptl1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat spleen tissue lysate)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Angptl1 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Angptl1 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Related Product Information for anti-Angptl1 antibody
Angptl1 (angiopoietin-like 1), also known as angioarrestin, ARP1, ANGPT3 or previously known as angiopoietin 3 (ANG3), is a member of the angiopoietin-like family. It is highly expressed in adult tissues, particularly adrenal gland, thyroid, placenta and small intestine. Angptl1 exists as a disulfide-linked dimer and shares 45.1% identity with Ang-1 and 59% identity with Angptl2. Angptl1 consists of an N-terminus with a coiled-coil domain, potential glycosylation sites and a C-terminus with a fibrinogen-like domain. It is a secreted protein but does not function as a growth factor in endothelial cells. Angptl1 plays a distinct role in the regulation of angiogenesis; inhibiting proliferation, migration, tube formation and endothelial cell adhesion. To exert this inhibitory activity, Angptl1 is speculated to interact with a receptor on endothelial cells. In a wide variety of tumor tissues, Angptl1 expression is down-regulated suggesting that a major function of this protein involves its antiangiogenic properties.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 56kDa
NCBI Official Full Name
angiopoietin-related protein 1
UniProt Protein Name
Angiopoietin-related protein 1
Protein Family
UniProt Gene Name
ANGPTL1
UniProt Synonym Gene Names
ANG3; ANGPT3; ARP1; ANG-3
UniProt Entry Name
ANGL1_HUMAN
Uniprot Description
ANGPTL1:
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q25.2
Cellular Component: extracellular space
Molecular Function: receptor binding
Biological Process: transmembrane receptor protein tyrosine kinase signaling pathway
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Product Notes
The Angptl1 angptl1 (Catalog #AAA3005675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Angptl1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Angptl1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Angptl1 angptl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angptl1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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