Rabbit anti-Human SAP30 Polyclonal Antibody | anti-SAP30 antibody

SAP30, CT (SAP30, Histone deacetylase complex subunit SAP30, 30kD Sin3-associated polypeptide, Sin3 corepressor complex subunit SAP30, Sin3-associated polypeptide p30) (APC)

Cat. Num. AAA6345009

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAP30; Polyclonal Antibody; CT (SAP30; Histone deacetylase complex subunit SAP30; 30kD Sin3-associated polypeptide; Sin3 corepressor complex subunit SAP30; Sin3-associated polypeptide p30) (APC); Sin3-associated polypeptide p30; anti-SAP30 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SAP30 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SAP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-218 amino acids from the C-terminal region of human SAP30.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SAP30 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SAP30 antibody detected the SAP30 protein (arrow).)

Related Product Information for anti-SAP30 antibody

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq].

Product Categories/Family for anti-SAP30 antibody

Antibodies; Histones

References

Brandt, S., et al. Int. J. Biochem. Cell Biol. 42(9):1472-1481(2010). Viiri, K.M., et al. Mol. Cell. Biol. 29(2):342-356(2009). Sichtig, N., et al. Arch. Biochem. Biophys. 467(1):67-75(2007). Szafranski, K., et al. Genome Biol. 8 (8), R154 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506783
• NCBI GeneID: 8819
• NCBI Accession #: NP_003855.1
• NCBI GenBank Nucleotide #: NM_003864.3
• UniProt Accession #: O75446; D3DP38
• Molecular Weight: 23,306 Da
• NCBI Official Full Name: histone deacetylase complex subunit SAP30
• NCBI Official Synonym Full Names: Sin3A-associated protein, 30kDa
• NCBI Official Symbol: SAP30
• NCBI Protein Information: histone deacetylase complex subunit SAP30; sin3-associated polypeptide p30; 30 kDa Sin3-associated polypeptide; Sin3-associated polypeptide, 30kDa; sin3-associated polypeptide, 30 kDa; Sin3 corepressor complex subunit SAP30
• UniProt Protein Name: Histone deacetylase complex subunit SAP30
• Protein Family: SAP30-binding protein
• UniProt Gene Name: SAP30
• UniProt Entry Name: SAP30_HUMAN

Uniprot Description

SAP30: Involved in the functional recruitment of the Sin3- histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N- CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. Belongs to the SAP30 family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q34.1

Cellular Component: nucleoplasm; histone deacetylase complex

Molecular Function: protein binding; DNA binding; metal ion binding; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic