Rabbit anti-Rat Angiotensin II Type 1 Receptor Polyclonal Antibody | anti-AGTR1 antibody
Angiotensin II Type 1 Receptor (AT1)
Gene Names
AGTR1; AT1-R
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
SerumSerum
Synonyms
Angiotensin II Type 1 Receptor; Polyclonal Antibody; Angiotensin II Type 1 Receptor (AT1); Anti -Angiotensin II Type 1 Receptor (AT1); anti-AGTR1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Angiotensin II type 1 Receptor. Sequence is the same in all AT II receptor isoforms (Type 1a, 1b and 1c). No sequence homology with other G-protein coupled receptors. Species sequence homology
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Applicable Applications for anti-AGTR1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blot.
Dilution: Western Blot: 1:1000-1:5000 (ECL). Detected ~45kD band.
ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07G Control peptide/well.
Dilution: Western Blot: 1:1000-1:5000 (ECL). Detected ~45kD band.
ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07G Control peptide/well.
Immunogen
Synthetic peptide 10aa sequence the N-terminus, extracellular of rat AT II (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-AGTR1 antibody
Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). Major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359aa; AT1B/AT III, 359aa; and AT1C, 177aa). Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular. The C-terminus is inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene (chromosome x) encodes 363 aa protein. It is highly expressed in myometrium with lower levels in adrenal and fallopian tube. It is also expressed at high levels in fetal kidney and intestine. Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while C-terminus inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene (chromosome x) encodes 363aa protein. It is expressed at high levels in myometrium, fetal kidney and intestine. It is expressed at lower levels in adrenal and fallopian tube.
Product Categories/Family for anti-AGTR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,046 Da
NCBI Official Full Name
angiotensin II type 1 receptor
NCBI Official Symbol
AGTR1
NCBI Official Synonym Symbols
AT1-R
NCBI Protein Information
type-1 angiotensin II receptor; AT1; angiotensin II type 1 receptor; angiotensin II type-1 receptor
UniProt Protein Name
Type-1 angiotensin II receptor
Protein Family
UniProt Gene Name
AGTR1
UniProt Entry Name
AGTR1_SHEEP
Uniprot Description
Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure: Interacts with MAS1
Subcellular location: Cell membrane; Multi-pass membrane protein.
Post-translational modification: Carboxyl-terminal Ser or Thr residues may be phosphorylated.
Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Research Articles on AGTR1
1. Chronic mild hypoxemia increased binding of angiotensin receptors in brain stem nuclei, decreased spontaneous baroreflex gain, and increased chemoreflex responses to asphyxia in the fetus.
2. replacement of thyroid hormone in hypothyroid sheep fetuses can restore renal ANG II receptor and renin expression and secretion to normal
3. TX decreased both angiotensin II receptor subtype 1 (AT1) mRNA and protein levels in kidney and lung but not in the left ventricle.
4. Angiotensin via the AT1 receptor may mediate, in part, the hypertensive effects of cortisol in utero.
5. pressor and renal vasoconstrictor effects of ANG II during the first 6 weeks of postnatal life in lambs are elicited by activation of AT1 but not AT2 receptors.
6. Immunoreactive AT1 receptor was decreased in the left ventricle of nutrient restricted fetuses compared to control fetuses. AT1 & AT2 message & protein are not tightly coupled, pointing to post-transcriptional control points in the mid gestation NR fetus.
2. replacement of thyroid hormone in hypothyroid sheep fetuses can restore renal ANG II receptor and renin expression and secretion to normal
3. TX decreased both angiotensin II receptor subtype 1 (AT1) mRNA and protein levels in kidney and lung but not in the left ventricle.
4. Angiotensin via the AT1 receptor may mediate, in part, the hypertensive effects of cortisol in utero.
5. pressor and renal vasoconstrictor effects of ANG II during the first 6 weeks of postnatal life in lambs are elicited by activation of AT1 but not AT2 receptors.
6. Immunoreactive AT1 receptor was decreased in the left ventricle of nutrient restricted fetuses compared to control fetuses. AT1 & AT2 message & protein are not tightly coupled, pointing to post-transcriptional control points in the mid gestation NR fetus.
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Product Notes
The AGTR1 agtr1 (Catalog #AAA622051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Angiotensin II Type 1 Receptor (AT1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Angiotensin II Type 1 Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blot. Dilution: Western Blot: 1:1000-1:5000 (ECL). Detected ~45kD band. ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07G Control peptide/well. Researchers should empirically determine the suitability of the AGTR1 agtr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiotensin II Type 1 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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