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Western Blot (WB)
(Western Blot Analysis: MW marker (1) and 20ug of kidney membrane extract (2) probed with MBS658452)
Angiotensin II Type 1 Receptor Peptide
Angiotensin II Type 1 Receptor (AT1)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Angiotensin II Type 1 Receptor; Polyclonal Peptide; Angiotensin II Type 1 Receptor (AT1); Angiotensin II Type 1 Receptor peptide
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Angiotensin II Receptor (AT1).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 50% glycerol.
Applicable Applications for Angiotensin II Type 1 Receptor peptide
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: Western Blot: 1:1000-1:2000. Predicted Molecular Weight: ~41kD. Higher Molecular Weight species (~55kD) and intermediates also observed which reflect post-translational modification and dimerization of the receptor.
Dilution: Western Blot: 1:1000-1:2000. Predicted Molecular Weight: ~41kD. Higher Molecular Weight species (~55kD) and intermediates also observed which reflect post-translational modification and dimerization of the receptor.
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT1. Species Sequence Homology: chimpanzee, bovine, rhesus macaque, sheep, canine, guinea pig, rabbit, equine, wild boar, gerbil.
Product Type
Pab
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: MW marker (1) and 20ug of kidney membrane extract (2) probed with MBS658452)
Western Blot (WB)
(Western Blot Analysis: MW marker (1) and 20ug of kidney membrane extract (2) probed with MBS658452)
Immunohistochemistry (IHC)
(Immunohistochemical staining of rat brain nucleus tractis solitarus fixed with 100% methanol and stained with propidium iodide (red) and MBS658452 at 1:1000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of rat brain nucleus tractis solitarus fixed with 100% methanol and stained with propidium iodide (red) and MBS658452 at 1:1000 dilution.)
ELISA (EIA)
(Cellular ELISA: HEK293 cells expressing AT1 Receptor were treated with indicated concentrations of agonist (Angiotensin II) and probed with MBS658452 (1:1000 of a stock solution of 1ug/uL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).)
ELISA (EIA)
(Cellular ELISA: HEK293 cells expressing AT1 Receptor were treated with indicated concentrations of agonist (Angiotensin II) and probed with MBS658452 (1:1000 of a stock solution of 1ug/uL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).)
Testing Data
(Membrane ELISA: Lewis rat kidney membranes (5 μg/well) were treated with indicated concentrations of agonist (Angiotensin II) and antagonist (Losartan) and probed with MBS658452 (1:1000 of a stock solution of 1ug/ul) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).)
Testing Data
(Membrane ELISA: Lewis rat kidney membranes (5 μg/well) were treated with indicated concentrations of agonist (Angiotensin II) and antagonist (Losartan) and probed with MBS658452 (1:1000 of a stock solution of 1ug/ul) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).)
Related Product Information for Angiotensin II Type 1 Receptor peptide
The octapeptide circulatory hormone Angiotensin II is a critical regulator of cardiovascular homeostasis formed in blood, with dual function in tissues of the brain, kidney, heart, and vasculature as an autocrine/paracrine growth hormone1,2. The actions of angiotensin II are mediated by type one (AT1) and type two (AT2) angiotensin II receptors, members of the seven-transmembrane G-protein coupled receptor (GPCR) family. AT1 receptors couple predominantly with Gq/11 alpha subunits, and signal through phospholipases C, D, and A2, inositol phosphates, and calcium channels, as well as a variety of serine/threonine and tyrosine kinases2. AT1 expression is greatest in vascular smooth muscle, liver, kidney, adrenal cortex, brain, and lung, and is primarily responsible for the physiological effects of angiotensin II, including regulation of arterial blood pressure, electrolyte and water balance, thirst, hormone secretion, and renal function. The AT2 receptor displays approximately 30% identity to the AT1 receptor, and is highly expressed during embryonic development1,2. AT2 expression in adult tissue is limited to the adrenal medulla, brain, and reproductive tissues, but is upregulated in injured tissue following myocardial infarction, vascular injury, and wound healing. AT2 receptor signaling is not dependent on G- protein coupling or receptor phosphorylation, although coupling to Gia2 and Gia3 alpha subunits occurs in some tissues3. AT2 activation can stimulate tyrosine.
Product Categories/Family for Angiotensin II Type 1 Receptor peptide
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
angiotensin II type 1 receptor
Similar Products
Product Notes
The Angiotensin II Type 1 Receptor (Catalog #AAA658452) is a Peptide produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Angiotensin II Type 1 Receptor (AT1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Angiotensin II Type 1 Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: Western Blot: 1:1000-1:2000. Predicted Molecular Weight: ~41kD. Higher Molecular Weight species (~55kD) and intermediates also observed which reflect post-translational modification and dimerization of the receptor. Researchers should empirically determine the suitability of the Angiotensin II Type 1 Receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiotensin II Type 1 Receptor, Polyclonal Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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