Western Blot (WB)
(Western blot analysis of AMPK alpha 1 C-term antibodyin mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified pAb. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit AMPKa1 Polyclonal Antibody | anti-AMPKa1 antibody
AMPKa1 antibody
Applications
ELISA, Western Blot
Purity
AMPKa1 antibody was purified by affinity chromatography
Synonyms
AMPKa1; Polyclonal Antibody; AMPKa1 antibody; Polyclonal AMPKa1; anti-AMPKa1; catalytic alpha-1 chain; AMPKa-1; AMPKa 1; AMPK alpha-1 chain; 5'-AMP-activated protein kinase; anti-AMPKa1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
AMPKa1 antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer with 0.09% (W/V) sodium azide
Concentration
250 ug/ml (varies by lot)
Sequence Length
458
Applicable Applications for anti-AMPKa1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 0.25 ug/ml
WB: 0.5-2.5 ug/ml
WB: 0.5-2.5 ug/ml
Biological Significance
AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.
Cross-Reactivity
Human, Mouse
Immunogen
AMPKa1 antibody was raised in Rabbit using synthetic peptide selected from the C-terminal region of human AMPK alpha 1 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of AMPK alpha 1 C-term antibodyin mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified pAb. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of AMPK alpha 1 C-term antibodyin mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified pAb. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-AMPKa1 antibody
Rabbit polyclonal AMPKa1 antibody
Product Categories/Family for anti-AMPKa1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
AmpKa1
Similar Products
Product Notes
The AMPKa1 (Catalog #AAA5301441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPKa1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 0.25 ug/ml WB: 0.5-2.5 ug/ml. Researchers should empirically determine the suitability of the AMPKa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPKa1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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