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Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKB1 antibody (Ser182))

Rabbit AMPKB1 Polyclonal Antibody | anti-AMPKB1 antibody

AMPKB1 antibody (Ser182)

Applications
Western Blot, Immunohistochemistry, ELISA
Purity
AMPKB1 antibody was purified by affinity chromatography
Synonyms
AMPKB1; Polyclonal Antibody; AMPKB1 antibody (Ser182); Polyclonal AMPKB1; Anti-AMPKB1; 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; AAKB1; AMP-activated protein kinase beta subunit; AMPK; AMPK beta -1 chain; AMPK beta 1; AMPK subunit beta-1; AMPKb; HAMPKb; MGC17785; PRKAB1; protein kinase AMP-activated beta 1 non-catalytic subunit; protein kinase AMP-activated noncatalytic beta-1; anti-AMPKB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
AMPKB1 antibody (Ser182) detects endogenous levels of AMPKB1 only when phosphorylated at serine 182
Purity/Purification
AMPKB1 antibody was purified by affinity chromatography
Form/Format
Liquid; PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-AMPKB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
Biological Significance
AMPKB1 is a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
AMPKB1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human AMPKB1 around the phosphorylation site of serine 182 (S-S-SP-P-P) as the immunogen.
Preparation and Storage
Ships on blue ice.
Store at -20°C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKB1 antibody (Ser182))

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKB1 antibody (Ser182))

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells. Left: Using AMPKbeta1 (phospho-Ser182) antibody. Right: Using the same antibody preincubated with synthesized peptide.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells. Left: Using AMPKbeta1 (phospho-Ser182) antibody. Right: Using the same antibody preincubated with synthesized peptide.)
Related Product Information for anti-AMPKB1 antibody
Rabbit Polyclonal AMPKB1 antibody (Ser182)
Product Categories/Family for anti-AMPKB1 antibody

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Product Notes

The AMPKB1 (Catalog #AAA5300847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPKB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:4000. Researchers should empirically determine the suitability of the AMPKB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPKB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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