Rabbit AMPK alpha 1 Polyclonal Antibody | anti-AMPKalpha1 antibody

Phospho-AMPK alpha 1 (Thr183)/AMPK alpha 2 (Thr172) Rabbit pAb

Cat. Num. AAA4755313

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human, Mouse, Rat, Monkey, Pig
• Applications: Immunocytochemistry, Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: AMPK alpha 1; Polyclonal Antibody; Phospho-AMPK alpha 1 (Thr183)/AMPK alpha 2 (Thr172) Rabbit pAb; PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK; anti-AMPKalpha1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey, Pig
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-AMPKalpha1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKalpha1/2 protein only when phosphorylated at T183/172.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-AMPKalpha1 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), ELISA (EIA)
• Application Notes: IF: 1:50-200; WB: 1:500-1:2000; IHC: 1:100-1:300; ELISA: 1:40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
• Conjugation: Unconjugated
• Modification: Phosphoralated
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of various cells using Phospho-AMPKalpha1/2 (T183/17,2) Polyclonal Antibody diluted at 1:500)

Western Blot (WB)

(Western Blot analysis of KB cells using Phospho-AMPKalpha1/2 (T183/17,2) Polyclonal Antibody diluted at 1:500)

Testing Data

(The picture was kindly provided by our customer)

Testing Data

(The picture was kindly provided by our customer)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree ,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree ,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK alpha (Phospho-Thr17,2) Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr17,2) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr17,2) Antibody. The lane on the right is blocked with the phospho peptide.)

Related Product Information for anti-AMPKalpha1 antibody

Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Effect of metformin on human periodontal ligament stem cells cultured with polydopamine?templated hydroxyapatite

Product Categories/Family for anti-AMPKalpha1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 27469582
• NCBI GeneID: 5562
• UniProt Accession #: Q13131; P54646
• Molecular Weight: 64,009 Da
• NCBI Official Full Name: PRKAA1 protein
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 1 catalytic subunit
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN