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Western Blot (WB)
(Western blot analysis of extracts of C2C12 cells using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:2000. C2C12 cells were treated by Oligomycin (0. 5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0. 5mM) for 30 minutes after serum-starvation overnight.)
Rabbit Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody | anti-PRKAA1 antibody
Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
Phospho-PRKAA1 (T183)/PRKAA2 (T172); Polyclonal Antibody; Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody; AMPKa1/AMPKa2; anti-PRKAA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-PRKAA1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
A phospho specific peptide corresponding to residues surrounding T172 of human AMPKalpha
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of C2C12 cells using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:2000. C2C12 cells were treated by Oligomycin (0. 5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0. 5mM) for 30 minutes after serum-starvation overnight.)
Western Blot (WB)
(Western blot analysis of extracts of C2C12 cells using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:2000. C2C12 cells were treated by Oligomycin (0. 5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0. 5mM) for 30 minutes after serum-starvation overnight.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat liver using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat liver using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat brain using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat brain using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human tonsil using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human tonsil using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human placenta using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human placenta using Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-PRKAA1 antibody
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed./The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Product Categories/Family for anti-PRKAA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,009 Da
NCBI Official Full Name
PRKAA1 protein
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
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Product Notes
The PRKAA1 prkaa1 (Catalog #AAA2566920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PRKAA1 (T183)/PRKAA2 (T172) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PRKAA1 (T183)/PRKAA2 (T172) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the PRKAA1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PRKAA1 (T183)/PRKAA2 (T172), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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