Rabbit AMPKalpha1/2 Polyclonal Antibody | anti-AMPKalpha1/2 antibody

AMPKalpha1/2 (Phospho-T183/172) Polyclonal Antibody

Cat. Num. AAA3005470

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AMPKalpha1/2; Polyclonal Antibody; AMPKalpha1/2 (Phospho-T183/172) Polyclonal Antibody; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; au-protein kinase PRKAA1; PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; PRKAA2; AMPK; AMPK2; anti-AMPKalpha1/2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-AMPKalpha1/2 (T183/172) polyclonal antibody detects endogenous levels of AMPKalpha1 protein only when phosphorylated at Thr183 and AMPKalpha2 protein only when phosphorylated at Thr172.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 550

• Applicable Applications for anti-AMPKalpha1/2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human AMPKalpha1 around the phosphorylation site of Threonine 183.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-AMPKalpha1/2 (T183/172) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-AMPKalpha1/2 antibody

AMPK is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory beta and gamma subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 27469582
• NCBI GeneID: 5562
• UniProt Accession #: Q13131; P54646
• Molecular Weight: ~ 63kDa
• NCBI Official Full Name: PRKAA1 protein
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]