Rabbit AMP Activated Protein Kinase beta1 Polyclonal Antibody | anti-Prkab2 antibody

AMP Activated Protein Kinase beta1, phosphorylated (Ser182) (5'-AMP-activated Protein Kinase Subunit beta-1, AMPK Subunit beta-1, AMPKb, PRKAB1, AMPK)

Cat. Num. AAA627924

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AMP Activated Protein Kinase beta1; Polyclonal Antibody; phosphorylated (Ser182) (5'-AMP-activated Protein Kinase Subunit beta-1; AMPK Subunit beta-1; AMPKb; PRKAB1; AMPK); Anti -AMP Activated Protein Kinase beta1; anti-Prkab2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMPKb when phosphorylated at serine 182. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Prkab2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:4,000; Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human AMPK beta1 around the phosphorylation site of serine 182 (S-S-SP-P-P).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Prkab2 antibody

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK is also involved in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP inhibits the dephosphorylation and inactivation of AMPK by PP2C. The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation. Phosphorylation at serine 108 of the beta1 subunit seems to be required for the activation of AMPK enzyme. Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization.

Product Categories/Family for anti-Prkab2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6013219
• NCBI GeneID: 64562
• UniProt Accession #: Q9QZH4
• Molecular Weight: 30,227 Da
• NCBI Official Full Name: AMP-activated protein kinase beta-2 regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• NCBI Official Symbol: Prkab2
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit beta-2; AMPK beta-2 chain; AMPK subunit beta-2; AMP-activated protein kinase beta-2 regulatory subunit
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit beta-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: Prkab2
• UniProt Synonym Gene Names: AMPK subunit beta-2
• UniProt Entry Name: AAKB2_RAT

NCBI Description

a component of the AMPK alpha2beta2gamma1 complex which phosphorylates glycogen synthase [RGD, Feb 2006]

Uniprot Description

AMPKB2: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity.

Protein type: Autophagy; Protein kinase, regulatory subunit

Cellular Component: nucleoplasm; apical plasma membrane; cytoplasm; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; identical protein binding; protein kinase binding

Biological Process: regulation of protein kinase activity; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process