Western Blot (WB)
(WB: Rat liver cell lysate was probed with anti-AMP Activated Protein Kinase alpha-pan)
Rabbit anti-Human, Rat AMP Activated Protein Kinase Polyclonal Antibody | anti-AMPK antibody
AMP Activated Protein Kinase, pan (AMPK) (MaxLight 750)
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AMP Activated Protein Kinase; Polyclonal Antibody; pan (AMPK) (MaxLight 750); anti-AMPK antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes AMP-activated protein kinase a subunits, Mr ~63kD. An unknown protein was also detected, Mr ~44kD, that was competed with the immunizing peptide. Species Crossreactivity: Rat and human.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-AMPK antibody
Western Blot (WB)
Application Notes
WB: 0.5-2microgram/ml detected AMP-activated protein kinase a subunits in a rat liver
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 4-22 of human AMPactivated protein a1 catalytic subunit (AEKQKHDGRVKIGHYILGD). The sequence shares 18/19 residues with rat and human AMP-activated protein kinase a2 catalytic subunit.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: Rat liver cell lysate was probed with anti-AMP Activated Protein Kinase alpha-pan)
Western Blot (WB)
(WB: Rat liver cell lysate was probed with anti-AMP Activated Protein Kinase alpha-pan)
Related Product Information for anti-AMPK antibody
(AEKQKHDGRVKIGHYILGD). The sequence shares 18/19 residues with rat and human AMP-activated protein kinase a2 catalytic subunit.
Product Categories/Family for anti-AMPK antibody
References
1. Kemp, B.E., et al., Trends in Biochem. 24: 22-25, 1999. 2. Michell, B.J., et al., J. Biol. Chem. 271: 28445-28450, 1996.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,009 Da
NCBI Official Full Name
PRKAA1 protein
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
Similar Products
Product Notes
The AMPK prkaa1 (Catalog #AAA6462786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMP Activated Protein Kinase, pan (AMPK) (MaxLight 750) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AMP Activated Protein Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-2microgram/ml detected AMP-activated protein kinase a subunits in a rat liver Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AMPK prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMP Activated Protein Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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