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Western Blot (WB) (TMED9 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the TMED9 antibody detected the TMED9 protein (arrow).)

Rabbit TMED9 Polyclonal Antibody | anti-TMED9 antibody

TMED9 Antibody (C-term)

Gene Names
TMED9; p25; GMP25; HSGP25L2G
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TMED9; Polyclonal Antibody; TMED9 Antibody (C-term); Transmembrane emp24 domain-containing protein 9; GMP25; Glycoprotein 25L2; p24 family protein alpha-2; p24alpha2; p25; GP25L2; anti-TMED9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TMED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human TMED9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
169-198
Sequence Length
235
Applicable Applications for anti-TMED9 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(TMED9 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the TMED9 antibody detected the TMED9 protein (arrow).)

Western Blot (WB) (TMED9 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the TMED9 antibody detected the TMED9 protein (arrow).)

Immunohistochemistry (IHC)

(TMED9 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TMED9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (TMED9 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TMED9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TMED9 antibody
The specific function of this protein remains unknown.
Product Categories/Family for anti-TMED9 antibody
References
Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004)
Zhang, H., et al. Nat. Biotechnol. 21(6):660-666(2003)
Zhang, H., et al. Nat. Biotechnol. 21(6):660-666(2003)
Renz, M., et al. J. Biol. Chem. 275(14):10429-10436(2000)
Wada, I., et al. J. Biol. Chem. 266(29):19599-19610(1991)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27277
NCBI Official Full Name
transmembrane emp24 domain-containing protein 9
NCBI Official Synonym Full Names
transmembrane emp24 protein transport domain containing 9
NCBI Official Symbol
TMED9
NCBI Official Synonym Symbols
p25; GMP25; HSGP25L2G
NCBI Protein Information
transmembrane emp24 domain-containing protein 9
UniProt Protein Name
Transmembrane emp24 domain-containing protein 9
UniProt Gene Name
TMED9
UniProt Synonym Gene Names
GP25L2; p24alpha2
UniProt Entry Name
TMED9_HUMAN

Uniprot Description

TMED9: Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer- dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: Golgi membrane; Golgi apparatus; trans-Golgi network transport vesicle; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane

Molecular Function: protein binding; syntaxin binding

Biological Process: COPI coating of Golgi vesicle; protein exit from endoplasmic reticulum; Golgi organization and biogenesis

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Product Notes

The TMED9 tmed9 (Catalog #AAA9211092) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 169-198. The TMED9 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TMED9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TMED9 tmed9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMED9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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