ALIX Polyclonal Antibody | anti-ALIX antibody

Anti-ALIX Antibody

Cat. Num. AAA177934

• Gene Names: PDCD6IP; AIP1; ALIX; HP95; DRIP4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ALIX; Polyclonal Antibody; Anti-ALIX Antibody; Programmed cell death 6-interacting protein; AIP1; ALG 2 interacting protein 1; ALG-2-interacting protein 1; ALG2 interacting protein X; Alix; Apoptosis linked gene 2 interacting protein X; Dopamine receptor interacting protein 4; DRIP4; Hp95; KIAA1375; MGC17003; PDC6I_HUMAN; PDCD6 interacting protein; PDCD6-interacting protein; PDCD6IP; Programmed cell death 6 interacting protein; programmed cell death 6 interacting protein; anti-ALIX antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 873

• Applicable Applications for anti-ALIX antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- ALIX Picoband antibody, MBS177934, Western blottingAll lanes: Anti ALIX (MBS177934) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: A375 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 96KDObserved bind size: 96KD )

Related Product Information for anti-ALIX antibody

Description: Rabbit IgG polyclonal antibody for Programmed cell death 6-interacting protein(PDCD6IP) detection. Tested with WB in Human;Mouse;Rat.

Background: Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

References

1. "Entrez Gene: PDCD6IP programmed cell death 6 interacting protein". 2. Vito P, Pellegrini L, Guiet C, D'Adamio L (Feb 1999). "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction". J Biol Chem 274 (3): 1533-40.

NCBI and Uniprot Product Information

• NCBI GI #: 241982780
• NCBI GeneID: 10015
• NCBI Accession #: NP_001155901.1
• NCBI GenBank Nucleotide #: NM_001162429.2
• UniProt Accession #: Q8WUM4; Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; C5MQH7; E9PFU1
• Molecular Weight: 30,453 Da
• NCBI Official Full Name: programmed cell death 6-interacting protein isoform 2
• NCBI Official Synonym Full Names: programmed cell death 6 interacting protein
• NCBI Official Symbol: PDCD6IP
• NCBI Official Synonym Symbols: AIP1; ALIX; HP95; DRIP4
• NCBI Protein Information: programmed cell death 6-interacting protein
• UniProt Protein Name: Programmed cell death 6-interacting protein
• UniProt Gene Name: PDCD6IP
• UniProt Synonym Gene Names: AIP1; ALIX; KIAA1375; PDCD6-interacting protein
• UniProt Entry Name: PDC6I_HUMAN

NCBI Description

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Uniprot Description

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: cytoplasm; cytosol; focal adhesion; immunological synapse; melanosome; membrane; microtubule organizing center; myelin sheath

Molecular Function: calcium-dependent protein binding; protein binding; protein homodimerization activity; proteinase activated receptor binding

Biological Process: apoptosis; cell separation during cytokinesis; mitotic metaphase plate congression; nuclear organization and biogenesis; protein transport; viral infectious cycle