Rabbit anti-Human PDCD6IP Polyclonal Antibody | anti-PDCD6IP antibody

PDCD6IP, ID (PDCD6IP, AIP1, ALIX, KIAA1375, Programmed cell death 6-interacting protein, ALG-2-interacting protein 1, Hp95)

Cat. Num. AAA649545

• Gene Names: PDCD6IP; AIP1; ALIX; HP95; DRIP4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PDCD6IP; Polyclonal Antibody; ID (PDCD6IP; AIP1; ALIX; KIAA1375; Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; Hp95); Anti -PDCD6IP; anti-PDCD6IP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PDCD6IP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: PDCD6IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-570 amino acids from the Central region of human PDCD6IP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in ZR-75-1 cell line lysates(35ug/lane) using MBS649545. This demonstrates that MBS649545 detected the PDCD6IP protein (arrow).)

Related Product Information for anti-PDCD6IP antibody

This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-PDCD6IP antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 241982780
• NCBI GeneID: 10015
• NCBI Accession #: NP_001155901.1
• NCBI GenBank Nucleotide #: NM_001162429.2
• UniProt Accession #: Q8WUM4; Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; E9PFU1
• Molecular Weight: 96,023 Da
• NCBI Official Full Name: programmed cell death 6-interacting protein isoform 2
• NCBI Official Synonym Full Names: programmed cell death 6 interacting protein
• NCBI Official Symbol: PDCD6IP
• NCBI Official Synonym Symbols: AIP1; ALIX; HP95; DRIP4
• NCBI Protein Information: programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; dopamine receptor interacting protein 4; apoptosis-linked gene 2-interacting protein X
• UniProt Protein Name: Programmed cell death 6-interacting protein
• Protein Family: Programmed cell death 6-interacting protein
• UniProt Gene Name: PDCD6IP
• UniProt Synonym Gene Names: AIP1; ALIX; KIAA1375; PDCD6-interacting protein
• UniProt Entry Name: PDC6I_HUMAN

NCBI Description

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Uniprot Description

Function: Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. Ref.10 Ref.11 Ref.16 Ref.21 Ref.22 Ref.23

Subunit structure: Interacts with SH3KBP1. Interacts with PDCD6 and TSG101 in a calcium-dependent manner. Interacts with and SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with HIV-1 p6. Interacts with EIAV p9; the interaction has been shown in vitro. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry. May interact with PDGFRB. Interacts with SH3GL1 and SH3GL2. Interacts with murine leukemia virus Gag polyprotein (via LYPX(n)L motif). Interacts with murine leukemia virus Gag polyprotein (via LYPX(n)L motif). Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15 Ref.17 Ref.18 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.27 Ref.29 Ref.32 Ref.33 Ref.34

Subcellular location: Cytoplasm › cytosol. Melanosome. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells. Ref.11 Ref.17 Ref.19 Ref.21 Ref.23

Post-translational modification: May be phosphorylated on tyrosine residues by activated PDGFRB. Ref.29

Sequence similarities: Contains 1 BRO1 domain.

Sequence caution: The sequence BAA92092.1 differs from that shown. Reason: Erroneous initiation.