Goat Pepsin ELISA Kit | PGA5 elisa kit

Goat Pepsin ELISA Kit

Cat. Num. AAA263098

• Reactivity: Goat
• Synonyms: Pepsin; Goat Pepsin ELISA Kit; PGA5 elisa kit

• Reactivity: Goat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 388

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PP concentration.
• Assay Type: Sandwich
• Detection Range: 2000 pg/ml-31.2 pg/ml
• Sensitivity: 12 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGA5 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat PP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 23943854
• NCBI GeneID: 5222
• NCBI Accession #: NP_055039.1
• NCBI GenBank Nucleotide #: NM_014224.3
• Molecular Weight: 41,993 Da
• NCBI Official Full Name: pepsin A-5 preproprotein
• NCBI Official Synonym Full Names: pepsinogen 5, group I (pepsinogen A)
• NCBI Official Symbol: PGA5
• NCBI Protein Information: pepsin A-5; pepsinogen-5; pepsinogen A5
• UniProt Protein Name: Pepsin A-5
• Protein Family: Pepsin
• UniProt Gene Name: PGA5
• UniProt Entry Name: PEPA5_HUMAN

NCBI Description

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

Uniprot Description

PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.

Protein type: Secreted; EC 3.4.23.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis