Rabbit anti-Human ALDH3B1 Polyclonal Antibody | anti-ALDH3B1 antibody

ALDH3B1, ID (Aldehyde Dehydrogenase Family 3 Member B1, Aldehyde Dehydrogenase 7, ALDH7) (Azide free) (HRP)

Cat. Num. AAA6462362

• Gene Names: ALDH3B1; ALDH4; ALDH7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ALDH3B1; Polyclonal Antibody; ID (Aldehyde Dehydrogenase Family 3 Member B1; Aldehyde Dehydrogenase 7; ALDH7) (Azide free) (HRP); anti-ALDH3B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH3B.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ALDH3B1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ALDH3B (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALDH3B1 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ALDH3B1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ALDH3B1 (arrow) using rabbit polyclonal ALDH3B1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH3B1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALDH3B1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ALDH3B1 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Marchitti, S.A., et.al., Biochem. Biophys. Res. Commun. 356(3), 792-798 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4502043
• NCBI GeneID: 221
• NCBI Accession #: NP_000685.1
• NCBI GenBank Nucleotide #: NM_000694.2
• UniProt Accession #: P43353; Q53XL5; Q8N515; Q96CK8; A3FMP9
• Molecular Weight: 51,840 Da
• NCBI Official Full Name: aldehyde dehydrogenase family 3 member B1 isoform a
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 3 family, member B1
• NCBI Official Symbol: ALDH3B1
• NCBI Official Synonym Symbols: ALDH4; ALDH7
• NCBI Protein Information: aldehyde dehydrogenase family 3 member B1; aldehyde dehydrogenase 7; aldehyde dehydrogenase 3B1
• UniProt Protein Name: Aldehyde dehydrogenase family 3 member B1
• Protein Family: Aldehyde dehydrogenase family
• UniProt Gene Name: ALDH3B1
• UniProt Synonym Gene Names: ALDH7
• UniProt Entry Name: AL3B1_HUMAN

NCBI Description

The aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular gene spans about 20 kb of genomic DNA and is composed of 9 coding exons. The gene is highly expressed in kidney and lung. The functional significance of this gene as well as the cellular localization of its product are presently unknown. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

ALDH3B1: Oxidizes medium and long chain saturated and unsaturated aldehydes. Metabolizes also benzaldehyde. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde. May not metabolize short chain aldehydes. May use both NADP(+) and NAD(+) as cofactors. May have a protective role against the cytotoxicity induced by lipid peroxidation. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.5; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - histidine; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasm; plasma membrane; cytosol; vesicle

Molecular Function: aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity

Biological Process: aldehyde catabolic process; ethanol catabolic process; alcohol metabolic process; lipid metabolic process