DCUN1D1 Antibody | anti-DCUN1D1 antibody

Goat anti-DCUN1D1, Biotinlyated Antibody

Cat. Num. AAA423743

• Gene Names: DCUN1D1; RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
• Reactivity: Human; Expected from sequence similarity: Mouse, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: DCUN1D1; Goat anti-DCUN1D1; Biotinlyated Antibody; Goat Anti-DCUN1D1 Antibody; DCN1; defective in cullin neddylation 1; domain containing 1 (S. cerevisiae); DCUN1L1; RP42; SCCRO; SCRO; Tes3; RP42 homolog; squamous cell carcinoma-related oncogene; anti-DCUN1D1 antibody

• Reactivity: Human; Expected from sequence similarity: Mouse, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 259

• Applicable Applications for anti-DCUN1D1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:16000.; WB: Approx 28kDa band observed in HepG2 lysates. Recommended concentration: 0.3-1ug/ml.
• Immunogen Sequence: Peptide with sequence C-RPQIAGTKSTT., from the C Terminus of the protein sequence according to NP_065691.2.
• Peptide Sequence + C: C-RPQIAGTKSTT.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

• NCBI GI #: 36030883
• NCBI GeneID: 54165
• NCBI Accession #: NP_065691.2
• NCBI GenBank Nucleotide #: NM_020640.3
• UniProt Accession #: Q96GG9; Q7L3G9; Q8TEX7; Q9H6M1; Q9HCT3; B2RB37
• Molecular Weight: 30.1kDa
• NCBI Official Full Name: DCN1-like protein 1 isoform 1
• NCBI Official Synonym Full Names: defective in cullin neddylation 1 domain containing 1
• NCBI Official Symbol: DCUN1D1
• NCBI Official Synonym Symbols: RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
• NCBI Protein Information: DCN1-like protein 1
• UniProt Protein Name: DCN1-like protein 1
• Protein Family: DCN1-like protein
• UniProt Gene Name: DCUN1D1

Uniprot Description

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression ().