Rabbit anti-Human ALDH2 Polyclonal Antibody | anti-ALDH2 antibody

ALDH2, ID (Aldehyde Dehydrogenase, Mitochondrial, EC 1.2.1.3, ALDH Class 2, ALDHI, ALDH-E2, ALDM) (PE)

Cat. Num. AAA6462335

• Gene Names: ALDH2; ALDM; ALDHI; ALDH-E2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ALDH2; Polyclonal Antibody; ID (Aldehyde Dehydrogenase; Mitochondrial; EC 1.2.1.3; ALDH Class 2; ALDHI; ALDH-E2; ALDM) (PE); anti-ALDH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ALDH2 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 318-347 in the central region of human ALDH2. UniProt Accession: #P05091.Species sequence homology: mouse, rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of 293 cell lysates (2ug/lane) using MBS629069 either nontransfected (Lane 1) or transiently transfected with the ALDH2 gene (Lane 2).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue using MBS629069 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-ALDH2 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Guo, Y.M., World J. Gastroenterol. 14(9), 1444-1449 (2008). 2. Chen, L., PLoS Med. 5(3), E52 (2008). 3. Teeguarden, J.G., Inhal Toxicol 20(4), 375-390 (2008). 4. Yoshida, A., Pharmacogenetics 2(4), 139-147 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 25777732
• NCBI GeneID: 217
• NCBI Accession #: NP_000681
• NCBI GenBank Nucleotide #: NM_000690.3
• UniProt Accession #: P05091; Q03639; Q6IB13; Q6IV71; B4DW54; E7EUE5
• Molecular Weight: 50,989 Da
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2 family member
• NCBI Official Symbol: ALDH2
• NCBI Official Synonym Symbols: ALDM; ALDHI; ALDH-E2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH2
• UniProt Synonym Gene Names: ALDM
• UniProt Entry Name: ALDH2_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2016]

Uniprot Description

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.2.1.3; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Mitochondrial; Carbohydrate Metabolism - propanoate; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrial matrix

Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity

Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process

Disease: Alcohol Sensitivity, Acute