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Western Blot (WB)

Rabbit GPD2 Polyclonal Antibody | anti-GPD2 antibody

GPD2 Antibody

Gene Names
GPD2; GDH2; GPDM; mGPDH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPD2; Polyclonal Antibody; GPD2 Antibody; GDH2; Glycerol 3 phosphate dehydrogenase 2 (mitochondrial); Glycerol 3 phosphate dehydrogenase 2; Glycerol 3 phosphate dehydrogenase mitochondrial; Glycerol-3-phosphate dehydrogenase; GPD 2; GPD M; GPD-M; gpd2; GPDH M; GPDH-M; GPDM; GPDM_HUMAN; mGPDH; mitochondrial; Mitochondrial glycerophosphate dehydrogenase; mtGPD; OTTHUMP00000162788; OTTHUMP00000204452; OTTHUMP00000204453; OTTHUMP00000204458; anti-GPD2 antibody
Ordering
 
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GPD2 antibody detects endogenous levels of GPD2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
727
Applicable Applications for anti-GPD2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human GPD2.
Subcellular Location
Mitochondrion.
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of mouse liver tissue, using GPD2 antibody.)

Immunohistochemistry (IHC)

(MBS9603634 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-GPD2 antibody
Function: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 68 kDa
Predicted: 81 kDa
NCBI Official Full Name
glycerol-3-phosphate dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
glycerol-3-phosphate dehydrogenase 2
NCBI Official Symbol
GPD2
NCBI Official Synonym Symbols
GDH2; GPDM; mGPDH
NCBI Protein Information
glycerol-3-phosphate dehydrogenase, mitochondrial
UniProt Protein Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
GPD2
UniProt Synonym Gene Names
GPD-M; GPDH-M

NCBI Description

The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]

Research Articles on GPD2

1. Observational study of gene-disease association. (HuGE Navigator)

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Product Notes

The GPD2 gpd2 (Catalog #AAA9603634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GPD2 gpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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