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Western Blot (WB) (Western blot analysis of ALB expression in HepG2 (A) whole cell lysates.)

Rabbit anti-Human ALB Polyclonal Antibody | anti-ALB antibody

Anti-ALB Antibody

Gene Names
ALB; HSA; PRO0883; PRO0903; PRO1341
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
ALB; Polyclonal Antibody; Anti-ALB Antibody; Serum albumin; anti-ALB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of ALB protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
609
Applicable Applications for anti-ALB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ALB. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of ALB expression in HepG2 (A) whole cell lysates.)

Western Blot (WB) (Western blot analysis of ALB expression in HepG2 (A) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ALB staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ALB staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-ALB antibody
Rabbit polyclonal antibody to ALB

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
213
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,160 Da
NCBI Official Full Name
serum albumin preproprotein
NCBI Official Synonym Full Names
albumin
NCBI Official Symbol
ALB
NCBI Official Synonym Symbols
HSA; PRO0883; PRO0903; PRO1341
NCBI Protein Information
serum albumin
UniProt Protein Name
Serum albumin
Protein Family
UniProt Gene Name
ALB
UniProt Entry Name
ALBU_HUMAN

NCBI Description

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor. [provided by RefSeq, Jul 2016]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; nucleus; protein complex

Molecular Function: chaperone binding; DNA binding; drug binding; fatty acid binding; identical protein binding; oxygen binding; protein binding; pyridoxal phosphate binding; toxin binding

Biological Process: bile acid and bile salt transport; cellular response to starvation; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; maintenance of mitochondrion localization; negative regulation of apoptosis; platelet degranulation; receptor-mediated endocytosis; retinal homeostasis; sodium-independent organic anion transport; transport

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

Research Articles on ALB

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Product Notes

The ALB alb (Catalog #AAA8248260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200. Researchers should empirically determine the suitability of the ALB alb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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