Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vitamin D3 receptor (VDR) Standard Curve.)
Rat Vitamin D3 Receptor (VDR) ELISA Kit | VDR elisa kit
Rat Vitamin D3 Receptor (VDR) ELISA Kit
Gene Names
Vdr; Nr1i1
Reactivity
Rat
Synonyms
Vitamin D3 Receptor (VDR); Rat Vitamin D3 Receptor (VDR) ELISA Kit; VDR; NR1I1; vitamin D (1; 25-dihydroxyvitamin D3) receptor; VDR elisa kit
Reactivity
Rat
Specificity
Rat Vitamin D3 receptor (VDR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VDR. No significant cross-reactivity or interference between Rat VDR and analogues was observed.
Sequence Length
423
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vitamin D3 receptor (VDR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vitamin D3 receptor (VDR) Standard Curve.)
Related Product Information for VDR elisa kit
Description: This Rat Vitamin D3 receptor (VDR) ELISA Kit employs a two-site sandwich ELISA to quantitate VDR in samples. An antibody specific for VDR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VDR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VDR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VDR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: VDR is the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.
Background: VDR is the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vitamin D3 receptor
NCBI Official Synonym Full Names
vitamin D receptor
NCBI Official Symbol
Vdr
NCBI Official Synonym Symbols
Nr1i1
NCBI Protein Information
vitamin D3 receptor
UniProt Protein Name
Vitamin D3 receptor
Protein Family
UniProt Gene Name
Vdr
UniProt Synonym Gene Names
Nr1i1; VDR
NCBI Description
binds vitamin D3; acts as a transcriptional regulator of the osteocalcin gene [RGD, Feb 2006]
Uniprot Description
Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Research Articles on VDR
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Product Notes
The Rat VDR vdr (Catalog #AAA9711610) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711610 ELISA Kit recognizes Rat VDR. It is sometimes possible for the material contained within the vial of "Vitamin D3 Receptor (VDR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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