Rabbit AKAP8 Polyclonal Antibody | anti-AKAP8 antibody

AKAP8 Antibody

Cat. Num. AAA9407173

• Gene Names: AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: AKAP8; Polyclonal Antibody; AKAP8 Antibody; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; AKA8; AKAP 95; AKAP95; anti-AKAP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total AKAP8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 692

• Applicable Applications for anti-AKAP8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human AKAP8.
• Target Name: AKAP8
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from mouse heart cells and Jurkat cells, using AKAP8 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human heart tissue using AKAP8 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HUVEC cells, using AKAP8 antibody.)

Related Product Information for anti-AKAP8 antibody

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).Eide T., Exp. Cell Res. 238:305-316(1998).Grimwood J., Nature 428:529-535(2004).Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

Product Categories/Family for anti-AKAP8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5031579
• NCBI GeneID: 10270
• NCBI Accession #: NP_005849.1
• NCBI GenBank Nucleotide #: NM_005858.3
• UniProt Accession #: O43823
• Molecular Weight: 76,108 Da
• NCBI Official Full Name: A-kinase anchor protein 8
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 8
• NCBI Official Symbol: AKAP8
• NCBI Official Synonym Symbols: AKAP-8; AKAP95; AKAP 95; AKAP-95
• NCBI Protein Information: A-kinase anchor protein 8
• UniProt Protein Name: A-kinase anchor protein 8
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP8
• UniProt Synonym Gene Names: AKAP95; AKAP-8; AKAP 95
• UniProt Entry Name: AKAP8_HUMAN

NCBI Description

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Uniprot Description

AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family.

Protein type: Adaptor/scaffold; Mitochondrial; Cell cycle regulation; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; mitochondrion; membrane; condensed chromosome; female pronucleus; nucleus

Molecular Function: zinc ion binding; double-stranded DNA binding

Biological Process: mitosis; mitotic chromosome condensation; signal transduction