Western Blot (WB)
(Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 μg/mL.)
Rabbit AIFM3 Polyclonal Antibody | anti-AIFM3 antibody
AIFM3 Antibody
Gene Names
AIFM3; AIFL
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
AIFM3 Antibody is affinity chromatography purified via peptide column.
Synonyms
AIFM3; Polyclonal Antibody; AIFM3 Antibody; AIFL; Apoptosis-inducing factor 3; Apoptosis-inducing factor-like protein; apoptosis-inducing factor; mitochondrion-associated; 3; anti-AIFM3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
AIFM3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
605
Applicable Applications for anti-AIFM3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
AIFM3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human AIFM3.
Buffer
AIFM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
AIFM3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of human brain tissue using AIFM3 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of human brain tissue using AIFM3 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of AIFM3 in human brain tissue with AIFM3 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of AIFM3 in human brain tissue with AIFM3 antibody at 20 μg/mL.)
Related Product Information for anti-AIFM3 antibody
AIFM3 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
65,966 Da
NCBI Official Full Name
Apoptosis-inducing factor 3
NCBI Official Synonym Full Names
apoptosis-inducing factor, mitochondrion-associated, 3
NCBI Official Symbol
AIFM3
NCBI Official Synonym Symbols
AIFL
NCBI Protein Information
apoptosis-inducing factor 3; apoptosis-inducing factor like
UniProt Protein Name
Apoptosis-inducing factor 3
Protein Family
UniProt Gene Name
AIFM3
UniProt Synonym Gene Names
AIFL
UniProt Entry Name
AIFM3_HUMAN
Uniprot Description
AIFM3: Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential. Belongs to the FAD-dependent oxidoreductase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; EC 1.-.-.-; Apoptosis; Endoplasmic reticulum; Oxidoreductase
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; nucleus
Molecular Function: 2 iron, 2 sulfur cluster binding; FAD binding; metal ion binding; oxidoreductase activity
Biological Process: cell redox homeostasis
Research Articles on AIFM3
Similar Products
Product Notes
The AIFM3 aifm3 (Catalog #AAA151107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AIFM3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AIFM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the AIFM3 aifm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIFM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
