Typical Testing Data/Standard Curve (for reference only)
Human Apoptosis inducing factor 3 (AIFM3) ELISA Kit | AIFM3 elisa kit
Human Apoptosis inducing factor 3 (AIFM3) ELISA Kit
Gene Names
AIFM3; AIFL
Reactivity
Human
Synonyms
Apoptosis inducing factor 3 (AIFM3); Human Apoptosis inducing factor 3 (AIFM3) ELISA Kit; AIFM3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of AIFM3. No significant cross-reactivity or interference between AIFM3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AIFM3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AIFM3 elisa kit
Intended Uses: This AIFM3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanAIFM3. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: AIFM3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AIFM3 antibody and an AIFM3-HRP conjugate. The assay sample and buffer are incubated together with AIFM3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIFM3 concentration since AIFM3 from samples and AIFM3-HRP conjugate compete for the anti-AIFM3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AIFM3 from the sample, fewer sites are left to bind AIFM3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIFM3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AIFM3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AIFM3 antibody and an AIFM3-HRP conjugate. The assay sample and buffer are incubated together with AIFM3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIFM3 concentration since AIFM3 from samples and AIFM3-HRP conjugate compete for the anti-AIFM3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AIFM3 from the sample, fewer sites are left to bind AIFM3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIFM3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AIFM3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,966 Da
NCBI Official Full Name
apoptosis-inducing factor 3 isoform 3
NCBI Official Synonym Full Names
apoptosis-inducing factor, mitochondrion-associated, 3
NCBI Official Symbol
AIFM3
NCBI Official Synonym Symbols
AIFL
NCBI Protein Information
apoptosis-inducing factor 3; apoptosis-inducing factor like
UniProt Protein Name
Apoptosis-inducing factor 3
Protein Family
UniProt Gene Name
AIFM3
UniProt Synonym Gene Names
AIFL
UniProt Entry Name
AIFM3_HUMAN
Uniprot Description
AIFM3: Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential. Belongs to the FAD-dependent oxidoreductase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Oxidoreductase; EC 1.-.-.-; Endoplasmic reticulum; Apoptosis
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; nucleus
Molecular Function: 2 iron, 2 sulfur cluster binding; FAD binding; metal ion binding; oxidoreductase activity
Biological Process: cell redox homeostasis
Research Articles on AIFM3
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Product Notes
The Human AIFM3 aifm3 (Catalog #AAA7206301) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206301 ELISA Kit recognizes Human AIFM3. It is sometimes possible for the material contained within the vial of "Apoptosis inducing factor 3 (AIFM3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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