Rabbit AIF Polyclonal Antibody | anti-AIFM1 antibody

Anti-AIF antibody

Cat. Num. AAA176953

• Gene Names: AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: AIF; Polyclonal Antibody; Anti-AIF antibody; Apoptosis-inducing factor 1; mitochondrial; apoptosis-inducing factor; mitochondrion-associated; 1; AIFM1 antibody; Apoptosis inducing factor 1; mitochondrial antibody; Apoptosis inducing factor antibody; Apoptosis inducing factor; mitochondrion associated; 1 antibody; Apoptosis-inducing factor 1 antibody; CMTX4 antibody; COXPD6 antibody; Harlequin antibody; Hq antibody; mAIF antibody; MGC111425 antibody; MGC5706 antibody; PDCD 8 antibody; PDCD8 antibody; Programmed cell death 8(apoptosis inducing factor) antibody; Programmed cell death 8 antibody; Programmed cell death 8 isoform 1 antibody; Programmed cell death 8 isoform 2 antibody; Programmed cell death 8 isoform 3 antibody; Programmed cell death 8 isoform 5 antibody; Programmed cell death protein 8 antibody; Programmed cell death protein 8 mitochondrial antibody; Programmed cell death protein 8 mitochondrial precursor antibody; Striatal apoptosis inducing factor antibody; anti-AIFM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 613

• Applicable Applications for anti-AIFM1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human AIF (596-613aa EQHEDLNEVAKLFNIHED), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-AIF antibody, MBS176953, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-AIF antibody, MBS176953, IHC(P)IHC(P): Rat Cardiac Muscle Tissue )

Western Blot (WB)

(Anti-AIF antibody, MBS176953, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: K562 Cell LysateLane 4: HEPG2 Cell LysateLane 5: A431 Cell LysateLane 6: NIH3T3 Cell Lysate )

Related Product Information for anti-AIFM1 antibody

Description: Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.

NCBI and Uniprot Product Information

• NCBI GI #: 13431764
• NCBI GeneID: 9131
• UniProt Accession #: O95831; Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 26,033 Da
• NCBI Official Full Name: Apoptosis-inducing factor 1, mitochondrial
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome)
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8
• UniProt Entry Name: AIFM1_HUMAN

NCBI Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Apoptosis; Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; DNA binding; electron carrier activity; NAD(P)H oxidase activity

Biological Process: neuron differentiation; caspase activation; chromosome condensation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; apoptosis; positive regulation of apoptosis; cell redox homeostasis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome