Rabbit anti-Human AGXT2 Polyclonal Antibody | anti-AGXT2 antibody

AGXT2, CT (AGXT2, AGT2, Alanine-glyoxylate aminotransferase 2, mitochondrial, (R)-3-amino-2-methylpropionate-pyruvate transaminase, Beta-ALAAT II, Beta-alanine-pyruvate aminotransferase, D-AIBAT) (Biotin)

Cat. Num. AAA6272335

• Gene Names: AGXT2; AGT2; DAIBAT
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AGXT2; Polyclonal Antibody; CT (AGXT2; AGT2; Alanine-glyoxylate aminotransferase 2; mitochondrial; (R)-3-amino-2-methylpropionate-pyruvate transaminase; Beta-ALAAT II; Beta-alanine-pyruvate aminotransferase; D-AIBAT) (Biotin); Alanine--glyoxylate aminotransferase 2; (R)-3-amino-2-methylpropionate--pyruvate transaminase; D-AIBAT; anti-AGXT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AGXT2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-AGXT2 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion within aa437-463 from the C-terminal region of human AGXT2, conjugated to KLH (Q9BYV1).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AGXT2 Antibody (C-term) in Hela cell line lysates (35ug/lane). AGXT2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(AGXT2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-AGXT2 antibody

The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor.

Product Categories/Family for anti-AGXT2 antibody

Antibodies; Enzymes; Aminotransferase

References

1. Rodionov, R.N., et al. J. Biol. Chem. 285(8):5385-5391(2010). 2. Baker, P.R., et al. Am. J. Physiol., Cell Physiol. 287 (5), C1359-C1365 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 17432913
• NCBI GeneID: 64902
• UniProt Accession #: Q9BYV1; Q53FB4; Q53FY7; Q53G03; Q5W7Q1
• Molecular Weight: 57,156 Da
• NCBI Official Full Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• NCBI Official Synonym Full Names: alanine--glyoxylate aminotransferase 2
• NCBI Official Symbol: AGXT2
• NCBI Official Synonym Symbols: AGT2; DAIBAT
• NCBI Protein Information: alanine--glyoxylate aminotransferase 2, mitochondrial; beta-ALAAT II; beta-alanine-pyruvate aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase
• UniProt Protein Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• Protein Family: Alanine--glyoxylate aminotransferase
• UniProt Gene Name: AGXT2
• UniProt Synonym Gene Names: AGT2; AGT 2
• UniProt Entry Name: AGT2_HUMAN

NCBI Description

The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

Uniprot Description

AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

Protein type: EC 2.6.1.40; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.44; Transferase

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding

Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; glyoxylate metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination