Rabbit anti-Human, Mouse AGXT / SPT Polyclonal Antibody | anti-AGXT antibody

Rabbit Polyclonal to Human AGXT / SPT

Cat. Num. AAA248809

• Gene Names: AGXT; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: AGXT / SPT; Polyclonal Antibody; Rabbit Polyclonal to Human AGXT / SPT; Anti-AGXT / SPT Antibody (Internal) IHC-plus; AGXT1; AGT1; PH1; SPAT; TLH6; Human AGXT; SPT; anti-AGXT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.
• Purity/Purification: Protein A purified
• Form/Format: PBS with 0.09% (w/v) sodium azide

• Applicable Applications for anti-AGXT antibody: Immunohistochemistry (IHC) - Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC; IHC-Paraffin (10ug/ml); Western Blot (1:1000); Flow Cytometry (1:10-1:50); (Optimal dilution to be determined by the researcher)
• Target: AGXT / SPT
• Host Note: AGXT / SPT antibody was produced in Rabbit
• Immunogen Species: AGXT / SPT antibody was raised against Human
• Epitope: aa96-125
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage, store at -20°C.

Immunohistochemistry (IHC)

(Anti-AGXT / SPT antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(AGXT Antibody (Center) (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGXT Antibody (Center) for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(AGXT Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of AGXT Antibody (Center) in HepG2 cell line lysates (35 ug/lane). AGXT (arrow) was detected using the purified Pab.(8 ug/ml))

NCBI and Uniprot Product Information

• NCBI GI #: 4557289
• NCBI GeneID: 189
• NCBI Accession #: NP_000021.1
• NCBI GenBank Nucleotide #: NM_000030.2
• UniProt Accession #: P21549; Q53QU6
• NCBI Official Full Name: serine--pyruvate aminotransferase
• NCBI Official Synonym Full Names: alanine-glyoxylate aminotransferase
• NCBI Official Symbol: AGXT
• NCBI Official Synonym Symbols: AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
• NCBI Protein Information: serine--pyruvate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransferase; alanine--glyoxylate aminotransferase; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase
• UniProt Protein Name: Serine--pyruvate aminotransferase
• Protein Family: Serine--pyruvate aminotransferase
• UniProt Gene Name: AGXT
• UniProt Synonym Gene Names: AGT1; SPAT; SPT; AGT
• UniProt Entry Name: SPYA_HUMAN

NCBI Description

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]

Uniprot Description

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.44; EC 2.6.1.51; Transferase; Motility/polarity/chemotaxis; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; receptor binding; pyridoxal phosphate binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process

Disease: Hyperoxaluria, Primary, Type I