Mouse Mucin 4 (MUC4) ELISA Kit | MUC4 elisa kit

Mouse Mucin 4 (MUC4) ELISA Kit

Cat. Num. AAA2020851

• Gene Names: MUC4; ASGP; MUC-4; HSA276359
• Reactivity: Mouse
• Synonyms: Mucin 4 (MUC4); Mouse Mucin 4 (MUC4) ELISA Kit; ASGP; Mucin 4 Cell Surface Associated; Mucin 4; Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin; MUC4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 4 (MUC4).; No significant cross-reactivity or interference between Mucin 4 (MUC4) and analogues was observed.
• Sequence Length: 88

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.26ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 4 (MUC4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 4 (MUC4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 4 (MUC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 4 (MUC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 4 (MUC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 4 (MUC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MUC4 elisa kit

Tumor immunity; Infection immunity; Pulmonology

NCBI and Uniprot Product Information

• NCBI GI #: 3551823
• NCBI GeneID: 4585
• UniProt Accession #: Q99102; O95938; Q9GZM2; Q9GZV6; Q9H481; Q9H482; Q9H483; Q9H484; Q9H485; Q9H486; Q9H487; Q9H4D6
• Molecular Weight: 206,605 Da
• NCBI Official Full Name: mucin 4, partial
• NCBI Official Synonym Full Names: mucin 4, cell surface associated
• NCBI Official Symbol: MUC4
• NCBI Official Synonym Symbols: ASGP; MUC-4; HSA276359
• NCBI Protein Information: mucin-4
• UniProt Protein Name: Mucin-4
• Protein Family: Mucin
• UniProt Gene Name: MUC4
• UniProt Synonym Gene Names: MUC-4; ASGP; ASGP-1; ASGP-2
• UniProt Entry Name: MUC4_HUMAN

NCBI Description

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

Uniprot Description

MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: proteinaceous extracellular matrix; extracellular space; membrane; integral to plasma membrane; Golgi lumen; vesicle

Molecular Function: ErbB-2 class receptor binding; extracellular matrix constituent, lubricant activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; cell-matrix adhesion; post-translational protein modification