Rat Glycoprotein 130 (gp130) ELISA Kit | gp130 elisa kit

Rat Glycoprotein 130 (gp130) ELISA Kit

Cat. Num. AAA2700881

• Gene Names: IL6ST; CD130; GP130; CDW130; IL-6RB
• Reactivity: Rat
• Synonyms: Glycoprotein 130 (gp130); Rat Glycoprotein 130 (gp130) ELISA Kit; CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta; gp130 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycoprotein 130 (gp130).; No significant cross-reactivity or interference between Glycoprotein 130 (gp130) and analogues was observed.
• Sequence Length: 49

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.244ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycoprotein 130 (gp130) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycoprotein 130 (gp130) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gp130 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 130 (gp130). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein 130 (gp130). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein 130 (gp130), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein 130 (gp130) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1246098
• NCBI GeneID: 3572
• UniProt Accession #: P40189; Q5FC04; Q9UQ41; A0N0L4
• Molecular Weight: 96,276 Da
• NCBI Official Full Name: gp130, partial
• NCBI Official Synonym Full Names: interleukin 6 signal transducer
• NCBI Official Symbol: IL6ST
• NCBI Official Synonym Symbols: CD130; GP130; CDW130; IL-6RB
• NCBI Protein Information: interleukin-6 receptor subunit beta
• UniProt Protein Name: Interleukin-6 receptor subunit beta
• UniProt Gene Name: IL6ST
• UniProt Synonym Gene Names: IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130

NCBI Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

Uniprot Description

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases (PubMed:2261637). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed:19915009, PubMed:23294003). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (). Has a role in embryonic development (). Does not bind IL6 (PubMed:2261637). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (). Required for expression of TRPA1 in nociceptive neurons (). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (). Required for normal trabecular bone mass and cortical bone composition ().