Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 5ug/ml Rabbit Anti-Rat AGRN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ) )
Rabbit Agrin Polyclonal Antibody | anti-AGRN antibody
Polyclonal Antibody to Agrin (AGRN)
Immunohistochemistry: 5-50ug/mL
Immunocytochemistry: 5-50ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 5ug/ml Rabbit Anti-Rat AGRN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ) )
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 5ug/ml Rabbit Anti-Rat AGRN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ) )
Western Blot (WB)
(Western Blot: Sample: Recombinant AGRN, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant AGRN, Rat.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
NCBI and Uniprot Product Information
NCBI Description
may play a role in synapse development and regeneration [RGD, Feb 2006]
Uniprot Description
agrin: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
Protein type: Cell adhesion; Cell development/differentiation; Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q36
Cellular Component: basal lamina; basement membrane; cell junction; cell surface; cytosol; extracellular matrix; extracellular region; extracellular space; integral component of membrane; plasma membrane; proteinaceous extracellular matrix; sarcolemma; synapse
Molecular Function: acetylcholine receptor regulator activity; ATPase inhibitor activity; calcium ion binding; heparan sulfate proteoglycan binding; laminin binding; protein binding; sialic acid binding; transforming growth factor beta binding
Biological Process: G-protein coupled acetylcholine receptor signaling pathway; neuromuscular junction development; neurotransmitter receptor metabolic process; plasma membrane organization; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; protein heterotetramerization; receptor clustering; regulation of GTPase activity; regulation of protein amino acid phosphorylation; regulation of synaptic growth at neuromuscular junction; synaptic transmission; synaptogenesis
Research Articles on AGRN
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Product Notes
The AGRN agrn (Catalog #AAA2027261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Agrin (AGRN) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Agrin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC);Immunoprecipitation (IP). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-50ug/mL Immunocytochemistry: 5-50ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the AGRN agrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Agrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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